Hydrolases TRIZ Cases

Controlled pH incubation and enzymatic hydrolysis produce yeast extract with at least 7 wt.% glutamate and 10 wt.% ribonucleotides.

This case integrates phospholipid hydrolysis with chemical refining to improve yield and limit soapstock viscosity.

This case evaluates ancestral lipase sequences for surfactant resistance and detergency, supporting lower-cost cleansing enzyme searches.

Solid-phase synthesis loses purity as RNA strands lengthen; RNA ligase joins short segments for scalable non-natural RNA production.

Urethanases cleave polyurethane bonds under mild conditions for defined recycling products.

This case shows how R691A mutant Cas9 tunes guide-RNA interaction to improve RNP editing precision without sacrificing on-target efficiency.

A heterologous enzyme pathway in recombinant host cells enables scalable, high-purity mogroside V biosynthesis.

Electroporation introduces serA, serC, and serB genes, enabling cyanobacteria to produce L-serine from CO2 without costly separation.

Engineered guide RNA, donor nucleic acid, and NHEJ inhibition support precise HDR editing within AAV packaging limits.

A prime editing workflow inserts a tag sequence into genome DNA to identify and verify off-target candidates accurately.

A single vector links candidate enzymes, target sites, and identifiers for parallel screening with improved consistency.

This enzyme-catalyzed process converts sesaminol glucosides faster, using optimized conditions to reduce enzyme needs and production cost.

A genetically modified Mi.III antigen mouse model enables blood pressure testing to improve hypertension drug candidate selection.

Sequential detergent treatment, salt extraction, and chromatin pelleting enable sensitive, label-free nuclear proteome characterization.

Genome editing or transformation modifies protein kinase activity to improve phosphorus use and crop yield under deficiency.

SHARP uses engineered PcrA helicase and SSB to produce PCR-like amplicons up to 6000 base pairs without thermal cycling.

This case uses Cas9 nickase, reverse transcription, and paired DNA flaps to edit both strands while avoiding HDR limits.

In-cell reverse transcription from retron RNA produces abundant RT-DNA, addressing limited exogenous DNA and transformation efficiency.

This CRISPR case uses engineered Cpf1 complexes and guide RNAs to target DNA or RNA, supporting editing with reduced off-target effects.

RNA aptamers recruit endogenous effectors in compact CRISPR editors for single-AAV multiplexing.

Barcode-guided sequencing evaluates retron ncRNA, guide RNA, and nuclease combinations to identify optimal genomic editing systems.

Engineered antigen-specific immune cells reduce SUV39H1 activity to build memory, lower antigen thresholds, and improve response durability.

This case uses entropy correlations from normal mode analysis to guide Cas protein variants with improved activity and specificity.