Hydrolases TRIZ Cases

Synergistic fungal enzyme blends degrade lignin and cellulose into simple sugars without harsh chemical pretreatment.

MbpAgo prokaryotic Argonaute cleaves highly structured RNA at room temperature without interfering with endogenous eukaryotic RNAi pathways.

Deep eutectic solvents replace organic solvents to maintain enzyme stability and increase yield during lipoamino acid preparation.

CRISPR-mediated CD45 gene knockout prevents fratricide in CD45-targeted CAR T cells, enabling safe bone marrow ablation without severe side effects.

Digestible primers eliminate primer byproducts and mispriming errors during single-cell RNA sequencing workflows.

Modifying the CSLM gene reduces glycoalkaloid content while delaying budding, solving the trade-off between toxin reduction and sprout suppression.

Recombinant antibodies inhibit CD73 enzyme activity, blocking adenosine production to reverse immunosuppression in the tumor microenvironment.

A one-step nucleic acid detection assay combines isothermal amplification with CRISPR-Cas12a cleavage for rapid, instrument-free analysis.

Targeting Id3 expression in CAR-T cells via CRISPR/Cas9 knockout prevents xeno-GVHD while preserving anti-leukemia activity.

ML-driven guide design enables sensitive multiplexed CRISPR diagnostics without complex instrumentation.

Prime editor inserts sequences into DNA tape to measure elapsed time while preventing monomer deletions that compromise recording accuracy.

Inhibiting H19X-encoded non-coding RNAs via antagomirs or CRISPR addresses ineffective treatments by restoring muscle mass.

Transforming soybeans with the GmPAP2.1 gene boosts durability against Soybean mosaic virus by shifting from R-gene recognition to RNA silencing mechanisms.

Low lipid and lactose starting materials suppress bitterness, unpleasant odor, and time-dependent discoloration during enzymatic hydrolysis.

A Cas endonuclease and guide polynucleotide complex directs a donor polynucleotide with short homology arms to a targeted genomic locus.

Targeted amino acid substitutions in variant Cas12i2 polypeptides stabilize RNA guide binding, reducing off-target effects across varying temperatures.

RNase H degrades RNA tracks to resolve the trade-off between motor endurance and velocity, allowing particles to travel several micrometers.

Segmented ex vivo genetic modification of CD34+ cells reduces sickling risks while maintaining treatment precision through targeted HBG promoter editing.

A mutant algal strain upregulates urea carboxylase and desaturase transcripts to boost EPA content.

Segmented fusion proteins with soluble mediators enable precise temporal control of T cell responses, reducing cytokine storm toxicity.

Engineered guide RNA scaffold incorporates a reverse transcription primer binding site to enable in situ sequencing of spacer sequences.

Sequence-specific nuclease digestion removes non-target nucleic acids, enabling high-fidelity enrichment of low-abundance targets.

pJAT plasmids streamline CRISPR workflows by merging cloning steps into a single Gateway recombination event, boosting HDR efficiency.

A sequencing-based proteomics method uses CRISPR/Cas9 tagging to quantify protein expression and localization across a cell library.

Engineered immunoglobulin heavy chain alleles lacking CH1 domains enable production of functional heavy chain-only antibodies in transgenic animals.

Fusing zinc finger domains with FokI nuclease reduces off-target effects while maintaining high cleavage activity across diverse genomes.

Optimized GCH1 variants in recombinant microbes boost 5HTP yields by eliminating exogenous cofactor requirements.

CRISPR-Cas recombineering selects recombinant cells by cleaving non-integrated targets, eliminating selection markers and streamlining large sequence assembly.

Segmented endolysin formulations target Clostridium perfringens cell walls via enzymatic hydrolysis, reducing antibiotic resistance emergence.

Spatio-temporal expression of a mutated SOG1 gene increases HDR frequency and editing uniformity while reducing transformant chimerism.

An artificial intron in the AAV rep gene controls expression levels, preventing cytotoxicity during stable cell line establishment.

Targeted mutations at positions 6, 30, and 32 of the protease pro region resolve secretion bottlenecks to boost industrial enzyme yields.

A fungal autoinducible expression system uses quorum sensing to regulate gene expression via recombinase activation.