Hydrolases TRIZ Cases

Genetically modified allogeneic cells reduce complement-mediated inflammatory reactions by expressing tolerogenic factors to prevent immune rejection.

Chemical ligation joins nucleic acid segments using non-nucleotide linkers, reducing synthetic errors while improving manufacturing precision.

A nucleic acid-guided nuclease system depletes unwanted target sequences from single-stranded DNA libraries using complementary oligonucleotide hybridization.

Cas protein-gRNA complexes isolate target nucleic acid regions while exonuclease treatment removes unprotected sequences to minimize sample loss.

Polypeptide precursors enable rapid silk fibroin hydrogel formation under physiological conditions, avoiding cytotoxic crosslinkers.

Tandem microfiltration removes soluble and insoluble impurities from recombinant insulin, resolving fouling challenges.

GFAP and UCH-L1 protein assays replace head CT scans to eliminate radiation exposure while maintaining diagnostic accuracy.

A CRISPR-Cas assay device detects specific Single Nucleotide Polymorphisms using programmable guide sequences.

Targeted gene insertion via meganucleases into amplifiable loci reduces clone-to-clone variability and manufacturing costs.

Engineered recombinant microorganisms convert fatty aldehydes into fatty amines using exogenous biosynthetic enzymes.

Dual-guided short effector proteins maintain specificity and efficiency across high viscosity and metal chelating biological sample conditions.

Histone deacetylase inhibitors improve gene modification efficiency and cell survival in long-term hematopoietic stem cells by altering chromatin structure.

In situ adapter labeling captures double-strand breaks directly within cells, resolving off-target detection limits in genome editing workflows.

A CRISPR-Cas13a effector system detects target RNA molecules with attomolar sensitivity using guide RNAs and masking constructs.

A helper plasmid facilitates stable gene incorporation into the yeast genome through paired homologous recombination sequences.

Targeted gene expression modulation thickens the aleurone layer while maintaining germination rates and agronomic traits.

Embryogenesis-inducing polypeptides modulate maize microspores to form haploid embryos, bypassing time-consuming self-fertilization cycles.

Hairpin loop and Y adaptors prevent double-stranded bias, increasing sequencing throughput and accuracy.

A diagnostic assay detects galactosemia using a bacterially-derived sugar-phosphate phosphatase.

Engineered nickases resolve PAM constraints and double-strand break risks to expand targetable genome sequences.

Engineered B-GEn.1 and B-GEn.2 nucleases reduce off-target effects while maintaining high activity in mammalian cells.

Pyrobaculum calidifontis VA1 esterase converts hydroxycinnamic acid esters to amides, replacing organic solvent synthesis to enable natural product labeling.

Replaces radiation-based imaging with biochemical measurement of RNase 1 glycosylation status, resolving low sensitivity and invasiveness trade-offs.

Engineered microbial cells express specific dioxygenases to convert lignocellulosic biomass into chemical intermediates.

Suspended transglutaminase in a polyol-water mixture maintains microbiological stability and eliminates dust hazards inherent in powdered forms.

Splitting tracrRNA into separate segments reduces synthesis costs while maintaining functional integrity.

Guanidinobutyrase and agmatinase enzymes enable yeast selection using alternative nitrogen sources.

Deleting HCN1-4 genes in hiPSC-derived cardiomyocytes prevents ventricular arrhythmias while maintaining cardiac repair efficacy.

Redirecting carbon flow in genetically modified eukaryotic microorganisms produces essential amino acids and lipids, eliminating aerobic fermentation costs.

Split intein fragments reconstitute to cleave target proteins rapidly, eliminating slow rates and premature in vivo cleavage risks.

A digital warm-start CRISPR assay partitions a one-pot reaction into sub-nanoliter microreactions for sensitive viral detection.

Modifying host protein expression levels in tomato plants to induce resistance against root-knot nematodes.

Introducing a linear non-homologous DNA composition promotes error-prone end-joining repair to increase indel generation rates.

Engineered Lactococcus lactis bacteria detect Vibrio cholerae CAI-1 molecules via a hybrid receptor and genetic circuit, enabling rapid clinical intervention.

Removing specific RNase L cleavage sites from coding regions prevents mRNA degradation, resolving low yield issues in mammalian expression systems.

Genetically corrected iPSC-derived keratinocytes produce functional type VII collagen to restore epidermal integrity.

Integrating bioethanol and biogas units with enzymatic stillage treatment reduces energy consumption while increasing ethanol yields.

Restriction enzymes and transposases linearize circular DNA to differentiate it from degraded linear forms, resolving misinterpretation in disease detection.

CRISPR-Cas system directs specific and random mutations into plasmid genes for rapid enzyme variant generation.

Segmented CRISPR-Cas9 components confer phage immunity to Lactobacillus buchneri, resolving fermentation spoilage risks from invasive genetic elements.

Mutated T7 RNA polymerases incorporate fully modified nucleotides into RNA transcripts to boost aptamer production efficiency.

Electrophoretic force overcomes spacer stalling to enable rapid, cost-effective polynucleotide sequencing without amplification.

A tamoxifen-inducible Cas9 system uses ERT2 domains to control enzyme activity through chemical induction.

CRISPR/Cas12a nuclease cleaves long DNA regions into manageable fragments, reducing probe costs and PCR errors while improving capture completeness.

Optimized Cpf1 systems with Hammerhead and HDV-like ribozymes improve genome editing efficiency and stability while reducing off-target effects in plant cells.

Engineered PtNTT2 variants increase intracellular retention of unnatural base pairs, resolving stability trade-offs in expanded genetic alphabet synthesis.

Site-directed mutagenesis improves thermal stability, allowing the enzyme to maintain activity during feed processing and reduce phosphorous pollution.

Optimized Rhizopus promoter TATA box mutations resolve insufficient expression intensity to boost lactic acid productivity.

Magnesium chloride stabilizes dry protein deamidase against degradation, preventing caking and deliquescence during storage.

CRISPR-nickase targets endogenous repeats to delete kilobase-scale sequences without inserting exogenous recombinases.