Hydrolases TRIZ Cases

Pretreating crystallizable polymers with reactive agents alters their structure, boosting enzymatic degradation throughput for efficient plastic recycling.

CRISPR-Cas9 genomic editing targets Fel d 1 sequences to eliminate allergens, bypassing slow selective breeding processes.

Site-directed mutagenesis of SEX4 and LSF2 enzymes alters starch structure without hazardous chemicals, resolving safety and biomass trade-offs.

A complex recruits DNA polymerase to target sites for high-fidelity synthesis.

Expanded repeat variable diresidue codes target all four nucleotides at positions one to four, reducing off-target interactions and boosting molecular activity.

Recombinant polynucleotide sequence directs Kluyveromyces marxianus to synthesize specific astaxanthin stereoisomers via metabolic engineering.

A nucleic acid vector encodes a nuclease to cleave its own DNA after transgene expression.

A multiple target screening system identifies targeted genetic scissors with high specificity and activity.

Multimodal resin chromatography with arginine elutes vitamin K-dependent proteins under mild conditions, avoiding harsh treatments that degrade stability.

Oligosaccharides shield enzymes from denaturation, maintaining activity through sterilization and extended shelf life.

Amphiphilic polymer-coated fluorescent nanoparticles enable direct optical readout of enzymatic activity in aqueous media.

Liquid recombinant Candida rugosa lipase converts non-edible oils into biodiesel, eliminating costly enzyme immobilization and alkali catalyst pollution.

NapDNAbp systems record cellular history through programmable DNA changes, capturing stimulus strength and duration without disrupting cell function.

IRON MAN protein sequesters iron in aerial tissues, limiting pathogen growth while maintaining root nutrient uptake.

Specific amino acid substitutions in a phytase mutant enhance heat resistance, enabling stable enzyme function during high-temperature feed pelleting processes.

Replacing chemical induction with optogenetic switching resolves the trade-off between temporal precision and differentiation efficiency.

Engineered alkaline protease variants with targeted amino acid substitutions maintain high specific activity in liquid detergent formulations.

A yeast cell-based screening method targets proteases to the endoplasmic reticulum for interaction with substrates.

Ligating molecular inversion probes without circularization recovers discarded un-circularized signals to report structural variants.

A homogeneous fluorescent assay kit measures lipoprotein lipase activity using a BODIPY-based triglyceride substrate.

A 1-FEH enzyme hydrolyzes 1-kestose into sucrose and fructose within sugar solutions.

An ATP-hydrolyzing enzyme converts extracellular ATP to adenosine, reducing immunosuppression and improving response rates for cancer patients.

Granulating Rhizopus lipase with high-fat soybean powder creates a stable enzymatic preparation for industrial use.

Segmenting the 44-orf carrimycin gene cluster allows targeted modification to enhance yield and antibacterial activity.

Overexpressing specific plant kinases and phosphatases accelerates vegetative development in transgenic crops.

Immobilized Vaccinia capping enzyme reactor eliminates expensive cap analogues to reduce production costs while ensuring high capping efficiency.

Lipase catalyzes esterification of sugars with fatty acids, eliminating HCl corrosion and dehydration side reactions.

Measuring reduced EphA4 extracellular domain levels via ELISA provides a reliable diagnostic marker for neurological diseases with cognitive impairment.

Engineered ACC variants with BCCP mutations increase malonyl-CoA concentration in recombinant host cells.

A mutated fluorescent protein reporter construct restores expression upon successful homologous recombination to enable early detection of gene-editing events.

Aptamers bind skeletal Troponin I protein with high affinity to enable sensitive molecular detection of muscle damage.

Deoxyribonuclease in the detergent breaks down biofilm DNA, enabling stain removal at lower surfactant concentrations.

A CRISPR-Cas13a diagnostic system uses RNA masking constructs to detect target nucleic acids with attomolar sensitivity.

Use synthetic DNA templates to quantify off-target cleavage, resolving precision versus specificity trade-offs.

Machine learning guides mutations in Cas9 protein domains to boost editing activity while reducing experimental screening time.

Targeted SBEII gene knockouts via TALE nucleases boost amylose and resistant starch levels to address rapid glucose absorption risks.

Engineered aminoacyl-tRNA synthetases resolve low incorporation efficiency by enhancing reactivity with N-methyl substrates.

Attenuating argT and hisJ genes reduces lysine transporter activity, preventing amino acid uptake and boosting production yields.

CRISPR/Cas9 creates targeted double-strand breaks in plant genomic DNA to enable precise genetic modifications.

CRISPR effector system replaces complex qPCR instrumentation with portable biochemical detection, achieving attomolar sensitivity for point-of-care diagnostics.

Database mining with defined sequence motifs identifies novel halohydrin dehalogenases, expanding the enzyme repertoire available for biocatalysis.

A CRISPR-Cas9 system activates the RSPO2 gene using a specific sgRNA to upregulate Wnt signaling.

Exonuclease cleanup removes degraded 3' tails from lariat RNAs, restoring intron-exon junction integrity for accurate sequencing.

CRISPR/Cas9 editing overcomes inefficient conventional gene modification by enabling precise ddx27 knockout in zebrafish for disease modeling.

Engineered double-strand-break agents modify male fertility genes to create sterile plants, replacing labor-intensive detasseling with genetic systems.

Simultaneous knockout of PYL1, PYL4, and PYL6 breaks the growth-stress trade-off, raising rice yield by 25-31%.

Deleting repetitive sequences from the MHC locus resolves sequencing complexity and reduces immune rejection risks in cellular medicine.