Hydrolases TRIZ Cases

Modified Hafnia alvei phytase variants introduce specific amino acid substitutions and insertions to enhance enzyme activity.

Engineered cross-type nucleic acid targeting nucleic acids combine Cpf1 and Cas9 components into a single platform.

A linear donor DNA construct with a marker gene enables efficient cell enrichment during CRISPR-mediated genome editing.

CopyCatcher systems use guide RNAs to cut mutant alleles and leverage the homologous chromosome as a repair template.

Nitrilase catalyzes acrylonitrile hydrolysis to ammonium acrylate, eliminating petrochemical oxidation damage and reducing energy consumption.

Deleting miR155 and targeting its locus for CAR insertion prevents acute graft-versus-host disease while maintaining cancer treatment efficacy.

Segmented CRISPR arrays and UDG inhibitors resolve the trade-off between genome editing precision and system complexity in Lactobacillus crispatus.

Culture hematopoietic stem cells with mesenchymal lineage precursor cells and histone deacetylase inhibitors to achieve significant ex vivo expansion.

Specific adenosine deaminase mutations in adenine base editors reduce cytosine editing and RNA off-target effects for precise gene therapy applications.

Modified oligonucleotides with cleavage domains and thermostable RNase H enzymes reduce non-specific amplification during PCR.

Flow Assisted Mutation Enrichment uses FACS to isolate cells with double knockout mutations via marker gene ablation.

Overexpressing ITPK and VIP genes modifies inositol pyrophosphate levels, improving phosphorus-use efficiency under limited soil availability.

A sympathetic printed-matter uses hydrophilic substrate portions on a hydrophobic base to visualize hidden content through brightness differences upon wetting.

Engineered CoA-dependent pathways redirect carbon flux to produce adipic acid and hexamethylenediamine while overcoming the optimality principle.

Mechanical shear creates a stable inclusion body dispersion that prevents settling and aggregation during high pressure refolding, boosting yields above 30%.

Recombinant Candida rugosa lipase 1 hydrolyzes oil-water mixtures at low temperatures, preventing trans-fat conversion and reducing production costs.

Dried hot-start PCR composition stabilizes pyrophosphatase with polyols to prevent non-specific amplification during room temperature storage.

Non-replicating minicells eliminate biosafety risks from antibiotic resistance genes while rapidly reducing pesticide half-life.

Small Type V nucleases integrate genes into the albumin locus, avoiding off-target effects and vector dilution during organ growth.

Dummy guide RNA integration eliminates molecular cloning bottlenecks, enabling high-throughput analysis of native gene expression at single-base resolution.

The Lip3 promoter achieves uniform transgene expression in all plant tissues, resolving the contradiction between broad adaptability and reliable consistency.

A genome edited Beta vulgaris plant with modified PPO2 protein confers herbicide resistance through targeted amino acid substitution.

CRISPR gene editing disrupts regulatory elements to reduce toxic protein production, addressing off-target effects and genomic instability.

Reduced VirD5 activity in modified Agrobacterium enables transient expression of editing machinery, minimizing exogenous DNA integration into the plant genome.