Hydrolases TRIZ Cases

Anti-ST2 antibodies deplete effector regulatory T cells to enhance CD8+ T cell infiltration and inhibit tumor growth.

Deoxyuridine nucleotides in the aptamer loop enable uracil-DNA glycosylase cleavage to release blockage and activate synthesis at elevated temperatures.

Enzymatic pathways synthesize six-carbon aliphatic backbones using methyl ester shielded carbon chain elongation to overcome microbial optimality principles.

Transition metal complex catalyst induces racemization of (S)-pantolactone, enabling near-complete conversion of the starting material into the desired product.

Metagenomic sequencing accesses novel CRISPR enzymes from uncultivated microbes, resolving limited microbial availability to enhance editing specificity.

A ramp tag increases recombinant protein expression by recruiting and reusing tRNA molecules during translation.

Class 2 CRISPR-Cas systems integrate PAM recognition domains with guide RNAs to resolve specificity trade-offs and eliminate off-target DNA cleavage events.

RNA-guided nucleases Cas9 and Cpf1 introduce site-specific double-strand breaks in tomato genomes using guide RNAs for precise heterologous sequence integration.

Novel cytosine base editor variants reduce Cas9-independent off-target DNA editing while maintaining high on-target efficiency.

Purified enzymes replicate and separate sister circular DNAs in vitro, eliminating primer design and cyclization steps required by rolling circle methods.

RNA editing oligonucleotides recruit ADAR enzymes to deaminate target adenosines, correcting G>A mutations that disrupt essential protein parts.

Temperature-dependent chelating agents regulate enzyme activity to reduce non-specific primer binding and side products during PCR amplification.

Controlled maturation using specific microorganisms balances weight preservation with aroma concentration, resolving trade-offs between dry and wet aging.

Template-free DNA polymerases incorporate 3′-O-blocked nucleoside triphosphates to synthesize labeled polynucleotide probes.

Segmented donor DNA guides repair to reduce random integration and immunogenicity during gene therapy.

Metagenomic sequencing accesses novel CRISPR/Cas enzymes from uncultivated microorganisms to resolve specificity and efficiency limitations in gene editing.

Heterologous beta-ketoacyl-ACP synthase expression in cyanobacteria overcomes low native productivity limits for sustainable biofuel feedstocks.

Recombinant Escherichia coli strains express Sus scrofa myoglobin using Pel B and Omp A signal peptides to boost protein yield.

Segmenting the Rad51 protein allows inserting tags at residue 54, maintaining DNA repair reliability while enabling real-time monitoring.

Dimeric IgA molecules bind mutant KRAS proteins with high specificity, resolving poor targeting precision in conventional cancer therapies.

Alkaline pH conditions enable cellulolytic enzymes to hydrolyze unconverted starch, reducing Maillard product formation and increasing ethanol yield.

C-terminus targeting antibodies detect proteolyzed calcineurin fragments without binding full-length protein, enabling spatial mapping of neuropathology.

Adding proteinases to cell culture media reduces growth factor concentrations while maintaining physiological relevance.

Alanine substitutions at backbone-contacting residues lower non-specific affinity, reducing off-target cleavage while maintaining on-target activity.

Prime editing systems correct pathogenic CFTR mutations using guide RNAs, addressing limited therapy effectiveness for diverse genetic variants.

A chimeric polyesterase hybridizes LCC and Thermobifida fusca cutinase domains to degrade polyethylene terephthalate fibers.

B. stearothermophilus and B. licheniformis enzyme blend lowers viscosity while preventing residual insoluble starch buildup during secondary liquefaction.

MESA biosensors decouple sensing from endogenous signaling via modular ligand-binding and transcription factor domains.

Blocking oligonucleotide adaptors hybridize with nucleic acid overhangs to join polynucleotides while preventing unwanted adaptor dimer formation.

TALE-nucleases correct STAT3 mutations in hematopoietic stem cells, restoring isoform balance and resolving graft-versus-host disease risks.

Novel RNA polymerase III promoters enable efficient guide RNA transcription while minimizing unintended polypeptide expression.

MMEJ and NHEJ inhibitors redirect DNA repair toward homology-directed mechanisms, increasing gene insertion frequency and precision in CRISPR editing.

CRISPR HDR system knocks in large DNA fragments using optimized donor constructs, eliminating toxic NHEJ inhibitors while maintaining high precision.

ANXA3 biomarker enables sensitive hepatocellular carcinoma diagnosis while monoclonal antibodies inhibit tumor progression.

Bridge helix-modified Cas9 proteins reduce off-target effects by improving mismatch discrimination against non-target DNA sequences.

Cancer transition inhibitors target adipose-derived stromal cell markers to overcome treatment resistance in diverse cancer types.

Cpf1 and Cas13 protein-RNA complexes use guide RNAs to target viral DNA or RNA, eliminating infected cells without harming human host tissue.

Engineered SpCas9 variants with specific amino acid substitutions at positions 924, 929, and 930.

Engineered DNase variants degrade extracellular DNA to remove biofilm malodors while maintaining detergent stability.