Hydrolases TRIZ Cases

Overexpressing alkaline phosphatase in Saccharomyces cerevisiae lowers intracellular ATP levels to divert carbon flux toward ethanol production.

Transforming plant germ cell meiosis into a mitosis-like state using gene editing to produce gametes with consistent genotype and chromosome ploidy.

Surface-active materials mediate lipase attachment to amino-functionalized supports, eliminating animal proteins and crosslinkers that cause allergic reactions.

A florigen pathway toolkit modulates flowering signals to customize tomato shoot architecture.

Humanized antibodies bind interleukin-6 with high affinity to block gp130 activation.

Uridine kinase generates CMP from cytidine to prevent nucleotide inhibition, enabling cost-effective CMP-Neu5Ac synthesis.

Genetically modified host cells utilize heterologous phosphoketolase and phosphotransacetylase to synthesize acetyl-CoA derived compounds.

Site-directed mutagenesis at position 363 of xylanase HwXy110A yields variants that maintain activity in high-temperature industrial environments.

A high-specificity Cas9 nuclease variant fused with a tRNA guide RNA fusion that undergoes precise enzymatic processing to remove extra nucleotides.

Site-directed mutagenesis improves DNase storage stability and biofilm breakdown efficiency in laundry formulations.

A CRISPR screening system introduces mutations into pooled cell populations to enable rapid phenotypic assessment without clone generation.

A thermo-responsive switchable solvent enables simultaneous cell disruption, oil extraction, and product separation in a single system.

CRISPR-Cas9 genome editing enables precise mutation introduction into primary leukocytes.

Antibodies inhibit human ORF2 endonuclease activity while preserving APE1 function, resolving specificity trade-offs.

CRISPR editing of HEK293 cells removes TMED10 and MON2 genes to boost viral vector output while maintaining manufacturing simplicity.

Co-immobilized enzymes on robust solid supports convert uridine monophosphate and N-acetylglucosamine into UDP-GlcNAc.

CRISPR-Cas9 editing of specific PPO genes reduces polyphenol oxidase activity, extending banana shelf life while avoiding unintended genome disruptions.

Genome-edited birds express human IgG Fc in hepatocytes to accumulate therapeutic proteins in egg yolk, avoiding allergic reactions from non-human glycans.

A gRNA array merges multiple sgRNA cassettes into a single transcript processed by ribozymes to release individual guides.

Segmented Ref nuclease systems using RecA-mediated strand invasion achieve targeted double-stranded DNA cleavage without engineered recognition sites.

Fc-fused butyrylcholinesterase proteins utilize carrier fusion to boost production yields in CHO cells.

Endonuclease cleavage and adapter ligation streamline guide RNA library generation, reducing time and cost for genome editing applications.

Modified DNase enzymes degrade extracellular DNA matrices to eliminate persistent biofilm malodors from textiles.

Novel esterases with specific amino acid substitutions enhance polyethylene terephthalate depolymerization yields.

Dual-target CRISPR-Cas9 excises integrated HIV DNA and disrupts the CCR5 receptor to eliminate persistent viral reservoirs.

A single-tube method uses Cas9 cleavage and thermophilic ligation to prepare sequencing libraries directly.

A transgenic mouse model suppresses Mfn2 gene expression in dopaminergic neurons to replicate mitochondrial dysfunction.

A CRISPR base editor system introduces targeted mutations into yeast cells to generate diverse protein libraries.

Methylation protects internal type IIS restriction sites, eliminating sequence constraints and scar accumulation during large DNA assembly.

Phospholipase C enzyme reduces oil loss by converting stubborn phospholipids into separable components.

Specific amino acid substitutions in the TadA domain of SECURE-ABE variants minimize off-target RNA editing while preserving on-target DNA editing efficiency.

Enzymatic deacetylation extends hyaluronic acid half-life without reducing mechanical strength, enabling stable tissue engineering scaffolds.

ORACLE method prepares unbiased phage libraries using acceptor phages and counterselection systems.

ACC2 inhibitors eliminate non-transformed organelles, resolving low transformation efficiency in monocotyledons.

A Cas9 polypeptide paired with a guide nucleic acid and a PAMmer oligonucleotide enables specific binding to single-stranded target nucleic acids.

Modifying the IscR regulator overcomes biosynthetic pathway bottlenecks to increase biotin, lipoic acid, or thiamine production levels.

A nitrilase mutant uses peptide fragment displacement to boost catalytic activity and stereoselectivity.

An immobilized lipase composition utilizes an oil-in-water emulsion to enhance transesterification activity.

Rare-cutting endonucleases integrate transgenes via homologous recombination to correct gain-of-function mutations in monogenic disorders.

Genetically engineered hematopoietic cells with reduced CLL1 expression resist anti-CLL1 therapy.

Engineering a double-stranded DNA sequence with a guide RNA and a transcriptional roadblock maintains high repair template concentration at the cleavage site.

Exogenous PFTα or bFGF mediators reduce DNA damage and apoptosis to improve gene targeting frequencies in genetically modified cells.

Engineered phytase variants degrade inositol phosphates with enhanced thermal resilience for industrial feed processing.

Lyophilized urease precipitates calcium carbonate to stabilize sand in underground constructions.

AAV vectors deliver CRISPR components to engineer CAR-T cells, resolving exhaustion trade-offs that limit solid tumor therapy efficacy.

Detecting C481S mutations guides second-generation inhibitor selection to overcome treatment resistance in B-cell malignancies.

Thioesterase enzymes in cyanobacteria release fatty acids into culture media, eliminating costly cell mass harvesting steps.

CRISPR/Cas systems incorporate polynucleotide sequences into cellular DNA via homology-directed repair, creating stable temporal logs of internal stimuli.

A biodegradable hydrogel synthesized from citric acid oligomers and lipase B catalyst emits strong fluorescence without toxic dopants.