Hydrolases TRIZ Cases

A glycine-terminated polypeptide tag boosts recombinant nitrilase soluble expression for pharmaceutical synthesis.

Modified FCE enzymes improve RNA vaccine stability and scalability by optimizing capping efficiency.

Integrating digestion, end repair, and A-tailing into one step eliminates bead purification needs while enhancing quality control.

Collateral nuclease activity in a multiplex CRISPR assay detects Chlamydia trachomatis and Neisseria gonorrhoeae, eliminating expensive equipment requirements.

Guide RNA-target sequence pair libraries enable high-throughput in vivo detection of nuclease activity through indel frequency measurement.

Targeting a chimeric gene to nucleolar DNA via a polymerase I promoter boosts productivity while preventing heterochromatinization and recombination issues.

Segmented guide RNAs resolve synthesis yield versus purity trade-offs, improving stability and Cas9 cleavage efficiency.

Stabilizing single-stranded DNA with binding proteins improves HDR efficiency by preventing degradation during synthesis.

Co-expressing tagged fusion proteins with recombinases isolates un-tagged RAD51 or DMC1, eliminating costly proteolytic steps and frequent re-preparation.

A Type II CRISPR/Cas9 system from Faecalibaculum rodentium utilizes a ribonucleoprotein complex to recognize and cleave target DNA sequences.

CRISPR/Cas9 reduces RMS1 protein abundance in rice to create photosensitive male sterile lines with stable fertility conversion under varying light conditions.

Automated liquid handling systems use DNA probes to degrade abundant small noncoding RNA, reducing technician hands-on time and wasted sequencing reads.

Microbial fermentation digests solid chitin to yield chitosan and glucosamine, avoiding toxic chemical byproducts.

Co-expressing a cutinase mutant enhances membrane permeability to resolve inefficient intracellular protein secretion in Bacillus subtilis.

Stacked reagent layers on a wicking pad deliver solutions sequentially, eliminating manual washing steps.

Engineered Cpf1 polynucleotides use mutated direct repeats to direct CRISPR activity toward multiple genomic targets via a single crRNA molecule.

Immobilises haemoglobin on a gelling substrate via freeze drying, preventing denaturation while preserving conformable oxygen delivery.

Novel CRISPR nucleases reduce off-target effects and improve delivery profiles by utilizing alternative nuclease families with distinct PAM requirements.

A nucleic acid test kit hybridization solution containing a cationic polymer and non-ionic surfactant.

Targeted integration of exogenous nucleic acid molecules into super-enhancer sequences within the CHO cell genome to achieve high-level and stable expression of recombinant proteins.