Hydrolases TRIZ Cases

A glycine-terminated polypeptide tag boosts recombinant nitrilase soluble expression for pharmaceutical synthesis.

Modified FCE enzymes improve RNA vaccine stability and scalability by optimizing capping efficiency.

Integrating digestion, end repair, and A-tailing into one step eliminates bead purification needs while enhancing quality control.

Collateral nuclease activity in a multiplex CRISPR assay detects Chlamydia trachomatis and Neisseria gonorrhoeae, eliminating expensive equipment requirements.

Guide RNA-target sequence pair libraries enable high-throughput in vivo detection of nuclease activity through indel frequency measurement.

Targeting a chimeric gene to nucleolar DNA via a polymerase I promoter boosts productivity while preventing heterochromatinization and recombination issues.

Segmented guide RNAs resolve synthesis yield versus purity trade-offs, improving stability and Cas9 cleavage efficiency.

Stabilizing single-stranded DNA with binding proteins improves HDR efficiency by preventing degradation during synthesis.

Co-expressing tagged fusion proteins with recombinases isolates un-tagged RAD51 or DMC1, eliminating costly proteolytic steps and frequent re-preparation.

A Type II CRISPR/Cas9 system from Faecalibaculum rodentium utilizes a ribonucleoprotein complex to recognize and cleave target DNA sequences.

CRISPR/Cas9 reduces RMS1 protein abundance in rice to create photosensitive male sterile lines with stable fertility conversion under varying light conditions.

Automated liquid handling systems use DNA probes to degrade abundant small noncoding RNA, reducing technician hands-on time and wasted sequencing reads.

Microbial fermentation digests solid chitin to yield chitosan and glucosamine, avoiding toxic chemical byproducts.

Co-expressing a cutinase mutant enhances membrane permeability to resolve inefficient intracellular protein secretion in Bacillus subtilis.

Stacked reagent layers on a wicking pad deliver solutions sequentially, eliminating manual washing steps.

Engineered Cpf1 polynucleotides use mutated direct repeats to direct CRISPR activity toward multiple genomic targets via a single crRNA molecule.

Immobilises haemoglobin on a gelling substrate via freeze drying, preventing denaturation while preserving conformable oxygen delivery.

Novel CRISPR nucleases reduce off-target effects and improve delivery profiles by utilizing alternative nuclease families with distinct PAM requirements.

A nucleic acid test kit hybridization solution containing a cationic polymer and non-ionic surfactant.

Targeted integration of exogenous nucleic acid molecules into super-enhancer sequences within the CHO cell genome to achieve high-level and stable expression of recombinant proteins.

A bacterial and yeast host cell combination converts glycerol into ethanol during fermentation.

CRISPR RNA trans-splicing targets male embryos to eliminate incubation waste.

Thermolabile exonucleases degrade single-stranded DNA via enzymatic hydrolysis, resolving harsh sample treatment caused by high inactivation temperatures.

Novel esterases with specific amino acid substitutions enhance catalytic activity and thermostability.

Enzymes hydrolyze chlorophyll derivatives during degumming, preserving carotenoids and tocopherol while avoiding costly clay treatment.

Sequence-specific nucleases induce targeted DNA breaks in plant cells to enable precise genomic modification without foreign genetic material.

Inhibiting DNMT3A preserves developmental potential in modified immune effector cells, sustaining antitumor activity against solid tumors.

Cross T0 plants carrying CRISPR components with isogenic lines to distribute mutations across progeny, reducing screening time for rare edits.

Modified dsRNA agents silence metabolic disorder genes to avoid drug interactions.

Porcine stem cell media maintains pluripotency across passages, reducing cloning complexity and costs.

Engineered E. coli expresses a fusion protein of formamidase and phosphite dehydrogenase to decompose specific substrates.

Elevated sPLA2-IIA and BUN biomarkers identify patients requiring specific inhibitors to resolve inconsistent treatment outcomes.

CRISPR-Cas9 editing corrects RAG1 mutations at the endogenous locus, resolving immune dysregulation risks from lentiviral vectors.

A CRISPR/Cas12a detection system uses guide RNA and fluorescent reporters to identify plant pathogens.

Editing the ANP32 gene eliminates viral replication factors, resolving vaccine efficacy limits caused by rapid viral diversity.

Corynebacterium humireducens naturally overproduces L-alanine, L-glutamic acid, and L-valine through efficient endogenous enzymes.

Engineered organophosphorus acid anhydrolase mutants degrade Sarin enantiomers with enhanced stereospecificity.

A multiplex nucleic acid detection system uses embedded solid support probes to capture amplified viral sequences directly during PCR amplification.

Segmented guide RNA components resolve the trade-off between system complexity and binding efficiency by establishing specific stem element configurations.

PestE esterase catalyzes amidation in aqueous buffers to produce hydroxycinnamic acid amides, enabling natural labeling for food and cosmetic applications.

Recombinant cells expressing Coleoptera and Orthoptera desaturases synthesize omega-3 LC-PUFAs, providing a sustainable alternative to fish-derived sources.

CRISPR/Cas9 edits CD34+ genomes to integrate stable FOXP3, preventing inflammatory conversion.

Overexpressing PP2CABA in transgenic plants promotes root suberization and lignification, resolving the trade-off between osmotic stress tolerance and yield.

Mutated phi29 DNA polymerase variants enhance thermal stability through specific amino acid substitutions.

Replacing corrosive fluorination chemistry with enzymatic catalysis to achieve high enantiomeric excess without Simulated Moving Bed chromatography.

Modified Bacillus host cell reduces biofilm components to increase polypeptide production efficiency.

Thermostable cellulase enzyme breaks down polysaccharides in flowback fluids, lowering viscosity and enabling fluid recycling.