Hydrolases TRIZ Cases

A gene editing system combining CRISPR nuclease, cytosine deaminase, and AP lyase to achieve precise short fragment deletions.

Diphosphomevalonate decarboxylase converts 3-hydroxyalk-4-enoates into 1,3-dienes through a direct biological reaction.

Single-bead SELEX selects stable aptamers that replace costly antibodies, lowering production expenses while maintaining diagnostic reliability.

Encapsulating enzymes in thermally responsive polymer shells prevents denaturation and retains catalytic activity at elevated temperatures.

Mutant endonuclease V enzymes selectively nick inosine-containing DNA sequences, reducing non-specific background signals and improving diagnostic accuracy.

NK cells with deleted EP2 and EP4 receptors overcome PGE2-mediated suppression to control metastatic disease.

A heat-resistant lipase catalyzes random transesterification in oils and fats to modify physical properties.

Synthetic pathway enzymes enable precise Argyrin production control through genetic manipulation of microorganisms.

CRISPR/Cas9 targets validated safe harbor loci to resolve the contradiction between high integration efficiency and unpredictable cellular disruption.

Engineered acetogenic cells convert waste carbon sources into acetone, reducing substrate costs and energy consumption.

Segmented RNAi compositions target toxic PPP2R5D alleles to treat Jordan's Syndrome without affecting wild-type function.

Measuring DUSP5 expression levels identifies suitable patients for DNA methyl transferase inhibitor treatments.

Humicola lanuginose lipase variants hydrolyze fats in aqueous systems without surfactants, reducing environmental harm from traditional organic solvents.

Nucleotidyl transferase enzymes synthesize polynucleotides using reusable initiators, eliminating toxic phosphoramidite waste and breakage.

Optimized modified polyribonucleotides encode Cas9 proteins to boost translational efficiency while minimizing immunogenicity in therapeutic applications.

CRISPR nuclease complexes use guide RNA to introduce double-strand breaks in HBV covalently closed circular DNA, disrupting viral replication.

Linking a nucleic acid sequence-recognizing module to a proteolysis tag reduces non-specific mutations by controlling complex persistence.

PRMT9 inhibition eradicates AML via type-I IFN response, improving long-term survival against recurrence.

Engineered genomic regions stack multiple disease resistance genes into single loci, reducing breeding time and yield drag.

An attenuated African swine fever virus strain uses an IPTG-inducible LacI-LacO system to control D1133L gene expression.

Inhibiting DNAse1L3 preserves nucleic acid immune triggers released by radiation therapy.

Dual sgRNAs guide SaCas9 to excise DMPK repeats, resolving low efficiency of prior nucleases and enabling precise therapeutic intervention.

CycA glycine transporter boosts threonine yield by improving precursor uptake, avoiding expensive auxotrophs.

Genetically modified HIPO cells eliminate HLA antigens and overexpress CD47 to prevent immune rejection in universal regenerative therapies.

Editing KMT2E at rs73184087 modulates histone methylation to treat pulmonary hypertension without dysregulated metabolic programs.

EDTA rescues and maintains flap endonuclease activity during storage, preventing loss of sensitivity.

A CRISPR/Cas chain reaction system amplifies detection signals through secondary crRNA activation cascades.

Reagent caps seal sample tubes to protect reactive components from humidity, extending shelf life while enabling autoclaving without sticking issues.

Thermally actuated probiotic cells release therapeutic proteins upon ultrasound heating.

A prime editing composition uses dsgRNA and chromatin-modulating peptides to enhance genome editing efficiency.

Overexpressing MEOX1 via CRISPRa generates smooth muscle cells rapidly, reducing production time and batch variability.

Stem-loop 2 mutations in variant guide RNA scaffolds strengthen Cas9 binding to resolve the trade-off between editing efficiency and genome-wide accuracy.

CLOuD9 uses dCas9 fusion proteins to reversibly juxtapose chromosomal loci, resolving the irreversibility bottleneck in chromatin structure manipulation.

A SidE effector family protein activates ubiquitin via ADP-ribosylation to enable ATP-independent conjugation.

Enzymatic cleavage of low-molecular-weight urethanes liberates aromatic amines and alcohols from polyether polyurethanes.

Metabolic engineering of prokaryotic hosts overcomes low titers in conventional fermentation, enabling industrial-scale production exceeding 100 g/L.

Internal protein tags preserve physiological function while enabling detection of molecular interactions through segmented bioluminescent complex formation.

Targeted bridge helix modifications improve DNA cleavage selectivity while managing protein structure stability.

TCF7 modulation generates persistent memory stem-like T cells that overcome inconsistent tumor-specificity and rapid exhaustion in solid tumor therapies.

Formulations containing isolated endonuclease 1 ribonucleases break down nucleic acids in organic stains and biofilms, improving stain removal and whiteness.

Cas12F nucleases employ single guide RNAs to achieve precise promoter deletions, eliminating the need for multiple guide RNAs and reducing system complexity.

Segmented zinc finger nucleases increase homologous recombination frequency by creating precise double-strand breaks at targeted genomic loci.

Ligand-responsive ribozymes provide spatial and temporal control over CRISPR activity, reducing off-target effects and germline mutation risks.

Targeted gene knockout of GGTA1, CMAH, and β4GalNT2 reduces immune rejection and calcification in xenotransplanted heart valves.

Artificial nucleic acid constructs bind specific Cas protein domains to inhibit enzyme activity and mitigate off-target effects in gene editing.

T4EndoVII and mismatch endonuclease cleave heteroduplex DNA at error sites, reducing sequence mismatches and indels during chemical synthesis.

Lyophilization removes water from enzyme solutions to create stable protein ionic liquids that maintain antigen recognition without refrigeration.