Hydrolases TRIZ Cases
- Expression Vector Signal Peptide Optimization for Protein Secretion
An expression vector uses a homologous signal peptide to direct protein export from host cells.
- Enzyme Removal via Organic Solvent Mixture
Organic solvent mixture removes residual enzyme activity from liquid reaction mixtures, avoiding thermal damage to phospholipids.
- Novel Extender Units for Polyketide Synthase Diversity
Novel extender units introduce hydroxyl or amino groups into polyketide backbones, expanding structural diversity beyond traditional malonyl-CoA precursors.
- Cas9 Surface Loop Mutations for Extended sgRNA Specificity
Surface loop mutations in Cas9 proteins disrupt 5' spacer interactions to expand target space and specificity without reducing activity.
- dCas13-RNase Fusion Proteins for Targeted RNA Cleavage
A dCas13-RNase fusion protein enables precise and programmable RNA cleavage through sequence-specific targeting.
- Cyclin D Genes Enhance iPS Cell Reprogramming
Cyclin D family genes boost induced pluripotent stem cell reprogramming efficiency without triggering tumorigenesis risks linked to c-Myc.
- ADAR-Mediated RNA Sensor for In Vivo Quantification
An ADAR-based RNA sensor system detects target molecules through duplex formation and enzymatic editing.
- Novel Hydrolase Protein for Hepatitis C Intermediate Synthesis
A novel hydrolase protein hydrolyzes dialkyl 2-vinylcyclopropane-1,1-dicarboxylate to produce optically pure intermediates.
- Multi-enzyme inositol synthesis from starch
A multi-enzyme reaction system converts starch or cellulose into inositol through sequential phosphorolysis and catalytic steps.
- Megasphaera elsdenii Metabolic Engineering for Longer Chain Alcohols
Genetically modified Megasphaera elsdenii increases carbon flux to butyryl-CoA, producing longer chain alcohols to address ethanol production limitations.
- Antibody-enzyme conjugate via hydrazide thiol linker
A molecular conjugate bonds an antibody to a detectable enzyme using a hydrazide thiol linker attached to the Fc region.
- Mutant Millet Lipase Reduction for Shelf Life
Mutant PgTAG Lipase genes remove rancidity from pearl millet flour by reducing lipase activity without complex processing.
- Sandwich Granule Enzyme Protection
Layered granules with PVA barriers maintain enzyme activity during steam pelleting.
- LPE18:1 Production via Pseudomonas Phospholipase A2
Phospholipase A2 treats Pseudomonas phospholipids to yield lysophosphatidylethanolamine 18:1, replacing egg yolk sources that lack this specific lipid.
- Phospholipase C Enzyme Safety and pH Stability
Non-pathogenic Aspergillus enzymes hydrolyze phospholipids without degrading phosphate esters, resolving safety concerns from pathogenic sources.
- Lipase Polypeptides for Harsh Cleaning Environments
Modified lipase polypeptides maintain activity in harsh composition environments to remove lipid stains.
- Split Mirrored Transposons for Nucleic Acid Contiguity Tracking
Segmented transposome complexes preserve contiguity data during fragmentation, enabling accurate reassembly of nucleic acid libraries.
- Engineered Retrons Enhance HDR Genome Editing
Engineered retrons produce msDNA to deliver donor templates, resolving intracellular concentration bottlenecks in HDR genome editing.
- Pol Theta Enhancement for Plant Genome Editing Insertion
Enhancing DNA polymerase theta activity promotes microhomology-mediated end joining pathways in plant cells.
- Targeted Insertion Sites in Maize Genome
Targeted insertion of DNA into specific genomic loci using site-specific nucleases to resolve expression stability issues.
- Plant Gene Editing via Hypoxic Treatment and ROS Scavenging
Hypoxic treatment and reactive oxygen species scavengers enhance plant gene editing frequency through improved homology-directed repair pathways.