Hydrolases TRIZ Cases

XB44L14 soybean uses molecular markers to accelerate breeding cycles while maintaining disease resistance and yield stability.

A conjugate molecule links CD39 and TGFβ inhibitory portions to block adenosine and TGFβ signaling pathways simultaneously.

Optimizing pH to 4.5–5.5 enables continuous extraction of secreted oils, reducing glycerin waste.

Cre-loxP recombination removes selection markers from homozygous knockout organisms, resolving the trade-off between modification detection and genome purity.

Duck retinoic acid-inducible gene I promoter drives avian RIG-I expression in chickens.

Occluding oligonucleotides block Cas13 access to structured target RNAs until strand displacement occurs.

An antisense RNA molecule suppresses GA20 oxidase_5 expression, reducing gibberellin levels to improve lodging resistance without off-types.

Homology-directed repair inserts broadly neutralizing antibody paratopes into B cell receptors to overcome pathogen antigenic variability.

CRISPR systems modify reproductive hormones in fish to induce genetic sterility.

CRISPR-Cas9 editing reduces EXN53 protein in yeast cell extracts, stabilizing nucleic acids against nuclease degradation and doubling luciferase activity.

Engineered lipase variants reduce odor generation while maintaining high wash performance in detergent applications.

Targeted frameshift mutations in seed storage proteins increase essential amino acid levels while avoiding abnormal plant phenotypes and regulatory hurdles.

Bonded carbonic anhydrase enzymes degrade calcium and magnesium ions into carbon dioxide, eliminating toxic regeneration chemicals.

Adding Aspergillus oryzae DNase during recovery breaks down recombinant DNA, ensuring protein purity and regulatory compliance.

Coffee-derived materials stabilize enzymes to eliminate residual stabilizers and off-flavors in soluble coffee.

Zinc finger nucleases excise transgenes from pollen via tissue-specific promoters, preventing ecological contamination.

Engineered microbial pathways convert substrates into high-purity ferulic acid, bypassing low-yield biomass degradation.

CRISPR-Cas editing of endogenous BG1 loci increases grain yield and drought tolerance in maize.

AVL101 nucleic acid vaccine merges multiple HPV antigens and adjuvants to overcome limited expression and immune evasion in therapeutic vaccines.

Adjusting pH below 5.5 and adding calcium chloride solubilizes over 80% of precipitates while maintaining enzyme stability.

Engineered nucleases inactivate endogenous diatom genes, enabling transformed cell selection without antibiotic resistance markers.

A catalytically inactive MAD7 nuclease variant binds target DNA sequences without cleavage activity.

Amycolatopsis enzyme variants replace zinc dependency to lower production costs and increase yield of [S,S]-EDDS.

Validating safe harbor loci prevents insertional mutagenesis while ensuring reliable transgene expression in gene therapy.

DeepSmallCas9 predicts small Cas9 activity from sequence data, resolving the trade-off between delivery efficiency and prediction accuracy.

Targeted lactonase gene removal in Candida bombicola eliminates unwanted ester bond formation, enabling 100% acidic sophorolipid production.

Segmented CRISPR gRNA targets BCL11A to increase fetal hemoglobin while maintaining targeting precision and minimizing off-target effects.

A reporter construct links nuclease activity to fluorescent signals for cell identification.

Peptidyl arginine deiminase modifies plant proteins via deamidation, resolving the trade-off between solubility and functional properties like gelling.

Integrating supramolecular gel into open-cell polymer foam pores anchors nanofibers to create a mechanically robust catalytic hydrogel.

Localizing phosphatases via a C-terminal motif overcomes ineffective carbon metabolism alteration in transgenic plants.

CRISPNA replaces unstable RNA guides with Peptide Nucleic Acids to eliminate off-target mutations and enhance binding specificity.

Overexpressing imine/enamine deaminase polypeptides catalyzes ammonia release from reactive intermediates in engineered host cells.

Engineered acyltransferases incorporate saturated medium-chain fatty acids into triacylglycerols.

Segmented hydrolytic bond stability in polymer particles prevents burst release and ensures sustained delivery of biologically active molecules.

Segmented ex vivo CRISPR editing of ZNF644 boosts fetal hemoglobin while managing cell culture complexity.

Editing embryonic stem cells with CRISPR before nuclear transfer reduces mosaicism and improves genetic modification efficiency in cattle and pigs.

Thermally stable phytases in transgenic maize break down phytic acid to release absorbable phosphate.

Transgenic plants accumulate storage lipids in vegetative tissues to increase protein supply while avoiding yield limitations of traditional sources.

CRISPR/Cas9 knockout of the G-LecRK-VI.13 gene eliminates a negative regulator, resolving insufficient plant resistance to root knot nematodes.

Replacing chromatography with sulfatase and tetrazolium salt reduces device complexity while maintaining measurement accuracy.

Sonication creates pores in pollen grains for direct RNP complex delivery, bypassing regeneration to produce homozygous plants.

Engineered nucleases target PD-1 and CTLA-4 genes to modify T cells, resolving exhaustion trade-offs.

Enzymatic carbon chain elongation synthesizes seven-carbon aliphatic backbones for industrial chemical production.

Separating Cas9 nickase and reverse transcriptase into independent RNA components reduces molecular size, enabling adeno-associated virus delivery.

Inducible promoters activate curing nucleases to eliminate prior editing vectors, preventing competition during recursive genome editing.

Combining CD39 and CD73 inhibitors blocks adenosine generation, resolving partial antibody inhibition of enzymatic activity.

Orthogonal synthetase and tRNA pairs incorporate fluorinated amino acids at selector codons, resolving structural perturbations in large proteins.