Hydrolases TRIZ Cases

Enzymes break down polysaccharide absorbent diaper cores into a liquified state, reducing landfill waste and enabling sewage disposal.

A closed TIL expansion workflow enables sterile culture, gene editing, and cryopreservation while reducing contamination, cost, and process time.

Encoded probe fragments replace complex amplification, enabling sensitive multiplex nucleic acid detection with lower background noise.

An RNA-guided nuclease fused to an error-prone polymerase broadens the editing window and enables targeted mutagenesis without library transformation.

Boosting pantothenate uptake and CoA synthesis helps recombinant microbes convert acetyl-CoA to isobutene with higher yield.

Pre-integrated antibody constant regions let editable cell lines accept new variable regions, cutting vector construction, clone selection, and production time.

Targeting the MATRILINEAL pPLAIIα gene raises plant haploid induction rates and speeds doubled haploid line development in maize breeding.

Potentiometric paper electrodes with CNT and r-GO enable rapid ARSA measurement in serum and whole blood without complex optical assays.

Probe-protected exoribonuclease analysis detects low-level ssRNA degradation by measuring released nucleotides while reducing intact-RNA background noise.

Engineered Cas and Tns protein complexes improve guide-directed nucleic acid integration and binding where conventional CRISPR lacks insertion capability.

Targeted CRN mutations increase corn kernel row number while preserving meristem organization, enabling transgene-free yield gains.

Prebuilt placeholder TCR cassettes let immune cells be rapidly retargeted with therapeutic receptors, reducing customization time for cancer therapy.

Hybrid DNA/RNA CRISPR guides use DNA targeting and RNA activation regions to resist RNase degradation and reduce off-target cleavage.

Nanoscopically dispersed enzyme-RHP complexes enable programmable polymer degradation, eliminating microplastics while avoiding enzyme leaching.

Combining adenovirus and herpesvirus replication genes in producer cells raises AAV vector yield beyond traditional helper-virus methods.

Targeted amino acid substitutions raise protease catalytic activity, thermal tolerance, acid resistance, and pH stability for industrial use.

Dual CD110 and CD117 antibody conditioning depletes endogenous stem cells for HSCT engraftment without toxic myeloablative regimens.

Targeted CRISPR-Cas edits in BRI1 fine-tune brassinosteroid signaling to deliver more consistent seed size and oil content gains.

Targeted HEPN1-HEPN2 mutations reduce Cas13a mismatch tolerance, enabling more accurate SNP and RNA variant detection.

Dual CRISPR-linked cytosine and adenine deaminases enable precise, reproducible DNA edits in plant cells at defined genomic loci.

Specific amino acid substitutions raise esterase activity and heat stability, enabling faster PET breakdown and monomer recovery for recycling.

Phosphatase treatment and adapter ligation improve double-strand break site sequencing, capturing target and off-target cuts with higher specificity.

CRISPR-Cas editing of the endogenous MAX1 gene improves branching, seed size, and seed number without transgenes, supporting higher yield.

Selective culture media and feeder conditions enable stable long-term passage of human XEN-like cells and derivation of TSCs and nPSCs.

Specific lipase substitutions plus polyamine microcapsules improve detergent wash performance, shelf life, and odor control.

A DNase and protease detergent blend breaks down biofilm DNA and proteins to limit redeposition, malodor, and whitening loss.

Reduced HLA expression, higher CD47, and O Rh-negative typing help pluripotent cells evade allogeneic rejection without HLA matching.

Direct ketone and formyl-CoA condensation in engineered microbes bypasses indirect C2/C3 intermediates to make branched products.

Modified Bacillus keratinases improve feather degradation activity, specificity, and stability to produce peptides and amino acids.

A transformer model links variable-length genomic context to tissue-specific expression and saliency-mapped regulatory regions for targeted gene editing.

Phospholipase D treatment improves high-protein liquid food texture by suppressing roughness and grittiness with a simpler enzymatic approach.

CRISPR-Cas13a with influenza-targeting crRNAs enables rapid, sensitive RNA detection and strain discrimination without RT-qPCR equipment.

Specific Bhr-PETase amino acid substitutions improve PET hydrolysis efficiency, making enzymatic recycling more practical.

Targeting the gibberellic acid pathway helps prevent male reproductive structures on female cannabis plants and preserves crop value.

Targeted gene disruption and enzyme overexpression redirect carbon flux in microbes to raise MEG and C3 yields while preserving metabolic balance.

AAV delivery enables in situ editing of native stem cells, avoiding ex vivo isolation, graft failure risk, and niche disruption.

A hairpin adaptor and modified dCTP preserve one DNA strand during deamination, enabling methylation mapping without losing sequence code.

Stepwise enal-cleaving and BVMO enzymes shorten terpene carbon skeletons under biocatalytic control to produce defined fragrance precursors.

CRISPR/Cas9 excises HTT exons to durably suppress huntingtin expression, avoiding the repeat dosing limits of RNA interference.

Targeting SlTPP4 replaces hormone-based parthenocarpy treatments, enabling seedless fruit set and stable fruiting under high-temperature stress.

Specific POLD1 amino acid mutations give plants robust geminivirus resistance, reducing disease severity while helping maintain crop yield.

Compact rAAV vectors use smaller CasX and CpG-reduced sequences to fit multiple gRNAs and improve targeted gene editing with lower immunogenicity.

Gene markers in PRR37, UPF2, and RAP2-7/TOE1 enable faster, more precise selection and editing of autoflowering Cannabis lines.

A compact Type V nuclease with a CCN PAM enables AAV delivery, high mammalian-cell editing activity, and lower off-target cleavage.

RNA aptamers tether deaminases to specific gRNAs, enabling multiplex point mutations with low crosstalk and fewer harmful byproducts.

Paired single-stranded nucleic acids form transposase binding sites to enable controlled sequence replacement without size limits.

CRISPR guide RNAs and inactive Cas regulators selectively raise or suppress plant cannabinoid pathways without permanent genome modification.

Engineered phospholipase C enzymes hydrolyze PC and PE in vegetable oils, cutting degumming oil loss while keeping diglycerides in the oil.

Thermostable mismatch endonuclease variants selectively cleave G-G, T-T, and G-T/T-G mismatches to improve PCR mutation detection and target DNA amplification.