Transient expression in plant seeds speeds dairy protein production while enabling high yield and cleaner purification with pH control and His-tag separation.
TBF1 transcription factor binds TL1 elements to reprogram gene expression through uORF sensing, resolving growth-to-defense transition bottlenecks.
Co-expressing Kex2p in Pichia pastoris converts pro-insulin into active two-chain insulin, avoiding trypsin-induced impurities.
Replacing dimethyl formamide with dioxane and sodium salts reduces des B impurities by 40% during human insulin methyl ester synthesis.