Library Linkers TRIZ Cases
- 5' Hairpin Adaptor Ligation for ssDNA-Specific Sequencing
A 5' hairpin adaptor and exonuclease-free polymerase enable ssDNA-specific library construction with low non-specific ligation and stable sequencing.
- 5′ Hairpin Adaptor Ligation for ssDNA-Specific Library Construction
Klenow 5′ end filling and renatured hairpin adaptor ligation separate ssDNA from dsDNA for sensitive sequencing library preparation.
- In Situ Probe Inversion via Click Chemistry
A probe inversion process circularizes oligonucleotides to convert 3'-bound probes to 5'-bound orientation.
- Clonal Pre-amplification in Emulsion for Bias-Free Nucleic Acid Analysis
Water-in-oil emulsion droplets isolate nucleic acid molecules for clonal pre-amplification via strand-displacement mechanisms.