Ligases TRIZ Cases
- Nucleotide Fragment Ligation for High-Purity Long Oligonucleotide Synthesis
Separately synthesized nucleotide fragments are hybridized and ligated to cut non-target strands and improve purity in long oligonucleotide synthesis.
- Rice ACCase Mutations for Synergistic Multi-Herbicide Resistance
Combined mutations in rice ACCase and other loci create synergistic resistance to ACCase and HPPD herbicides for stronger weed control.
- L-Amino Acid Ligase for Broad-Substrate Dipeptide Synthesis
A codon-optimized L-amino acid ligase expands substrate range and maintains high dipeptide synthesis efficiency at industrially useful concentrations.
- PylRS Enzyme Selection for High-Efficiency Non-Canonical Amino Acid Loading
Using highly concentrated MaPylRS improves non-canonical amino acid incorporation in E. coli and cell-free protein synthesis.
- Suppressor tRNA Evolution for Efficient Uaa Incorporation in Mammalian Cells
Virus-assisted directed evolution enriches active suppressor tRNAs, boosting unnatural amino acid incorporation and stable mammalian cell line generation.
- Site-Specific O-Sulfotyrosine Incorporation for Homogeneous Protein Sulfation
An engineered tRNA synthetase/tRNA pair inserts O-sulfotyrosine at UAG sites, overcoming heterogeneous sulfation during high protein expression.
- Synthetic Supercoiled Circular DNA Assembly Without Bacterial Sequences
HTLA joins precursor DNA in vitro to make supercoiled circular DNA without bacterial sequences, cutting production time and immune-response risks.
- ASNS-Auxotrophic Mammalian Cell Lines for Multi-Vector Selection
ASNS knockout mammalian cell lines use exogenous asparagine dependence to enable multi-vector selection for recombinant protein production.