Microorganism Based Processes TRIZ Cases

Targeted amino acid mutations improve enzyme activity and thermal stability, resolving low productivity and poor reliability in L-phosphinothricin synthesis.

Suppressing the pdhr transcription factor redirects metabolic flux toward c6 compounds while reducing valine by-product accumulation.

An alanine-substituted prephenate dehydratase variant boosts branched-chain amino acid yields by up to 1.5-fold while minimizing L-phenylalanine by-products.

Engineering Gram-positive bacteria with yvyD overexpression cassettes resolves unpredictable yield issues while maintaining simple cell construction.

Segmented recombinant proteins link distinct parasite epitopes to resolve diagnostic sensitivity and specificity trade-offs in resource-poor settings.

A site-specifically labeled transmembrane protein nanodisc uses cysteine mutation and chemical conjugation to achieve precise labeling.

Removing toxin-antitoxin system proteins prevents metabolic shutdown, increasing limonene titers to 760 mg/L without extensive strain engineering.

Engineered cells express glycosyltransferases to synthesize HMO blends with precise LNFP-I and 2'-FL ratios, eliminating individual purification steps.

Targeted amino acid substitutions in the YjeH inner membrane protein boost precursor export, resolving low yield bottlenecks in methionine production.

hrmA and baf proteins enable high citric acid yields under low aeration, eliminating complex air supply systems.

Yeast suspension cultures chemically modify cannabinoids into water-soluble glycoside compounds.

Position 192 proline-to-leucine substitution in asparagine synthase increases L-valine yield without complex gene removal.

A mutant alpha-L-fucosidase overcomes steric hindrance in Fc domains to efficiently remove core fucose and enhance therapeutic antibody ADCC activity.

Targeting B7-H3 on AML cells reduces toxicity from normal cell damage while improving remission rates.

Modifying native putative steroid dehydrogenase in filamentous fungal host cells resolves metabolic stability trade-offs while increasing polypeptide yield.