Nucleotide Libraries TRIZ Cases
- Hybrid NGS Library Preparation for ssDNA Recovery After Bisulfite Conversion
A hybrid dsDNA and ssDNA workflow preserves fragmented molecules after bisulfite conversion, improving methylation detection and multiomic analysis.
- Dual Nucleic Acid Indexing for Accurate Low-Diversity Sequencing
A dual-index pool assigns diverse index sequences to each sample, reducing misassignment and index hopping in low-diversity sequencing.
- Droplet Reagent Transfer Using Mediators for Reaction Rate Control
Mediator-driven reagent transfer between droplets adjusts concentrations to control reaction rates without complex droplet handling.
- Orthogonal Oligonucleotide Synthesis With Minimal Chemistry Cross Talk
Orthogonal DMTr and Fmoc protection with split-pool synthesis enables parallel oligonucleotide production while minimizing chemistry cross talk.
- Pig 10K mSNP Liquid-Phase Chip for Low-Cost Genomic Selection
Multiple-SNP probe design boosts effective marker density in a 10K pig liquid-phase chip, cutting genomic selection cost while preserving 50K-level utility.
- Genome Structural Variation Detection via Polymer Matrix Sequencing
Embedding cells in a functional polymer matrix enables high-resolution sequencing of heterogeneous genomic variations, including eccDNA.
- Single-Assay DNA and RNA Library Generation on PolyT Flowcells
PolyT capture probes and transposase tagmentation enable one assay to generate DNA and RNA libraries, cutting time, complexity, and reagent use.
- Murine Tumor Immune Gene Panel for Immunotherapy Response Prediction
A targeted 50-plus-gene murine tumor assay improves immune-response profiling and speeds immunotherapy response prediction.
- Porcine 50K mSNP Liquid Chip for Informative Low-LD Genotyping
By selecting moderate-LD mSNP regions around target loci, this porcine 50K liquid chip boosts marker informativeness and cuts haplotype analysis burden.
- Mixed-Enzyme DNA Adapter Ligation for Higher Sequencing Library Yield
Combining DNA ligase with T4 RNA ligase boosts double-stranded DNA adapter ligation and improves sequencing library yield with less adapter residue.
- Direct tRNA Sequencing With Splinted Oligos for Higher Read Yield
Pre-annealed splinted oligonucleotides replace gel purification to boost tRNA nanopore read yield, preserve RNA recovery, and support multiplexing.
- Esophageal Methylation Screening for Barrett's Dysplasia Detection
Methylated DNA markers from esophageal brushing samples improve detection of Barrett's dysplasia and adenocarcinoma without random biopsy.
- Concatenated Polynucleotide Sequencing to Reduce Nanopore Open Time
Sequentially joining target polynucleotides cuts nanopore open time, boosts throughput, and lowers pore blocking risk.
- Ultrashort cfDNA Extraction Using SPRI Beads for NGS Detection
SPRI beads and phenol:chloroform extraction retain ultrashort cfDNA below 100 bp, improving NGS library coverage and biomarker detection.
- DNA-Barcoded Bead Libraries for Single-Cell Picowell Screening
Bead-bound compounds with DNA barcodes enable single-bead picowell screening, controlled release, and precise single-cell response tracking.
- Multiplex Sequencing Library Prep With Closed-Tube Self-Normalization
Closed-tube multiplex amplification adds capture, indexing, and adapters in one reaction to cut contamination and avoid separate normalization.
- Loopable mRNA Translation for Stable Repetitive Protein Synthesis
Circularized mRNA with a self-splicing intron enables efficient repetitive protein synthesis while avoiding large unstable DNA constructs.
- Nucleic Acid Tag Libraries for Forgery-Resistant Product Authentication
Position-based nucleic acid motifs and randomized sequences make product tags far harder to sequence, copy, and forge.
- Flow Cell Spatial Indexing With Hydrogel Beads for Barcode-Free Sequencing
Hydrogel beads and a liquid diffusion barrier spatially seed libraries on a flow cell, removing barcoding and simplifying sequencing analysis.
- Primary Template Amplification for Uniform Nucleic Acid Sequencing
Terminator-guided strand displacement improves sequence representation and uniform coverage in small-sample nucleic acid amplification.
- Multiplex Sequencing Library for Endometrial Cancer Gene Mutation Detection
Modified degenerate primers enable single-tube sequencing library construction for eight endometrial cancer genes, cutting sample demand, time, and cost.
- Gene Panel Classification for Lung Adenocarcinoma Survival Risk
A lung adenocarcinoma gene panel uses expression profiling to classify molecular subtypes and survival risk for more personalized treatment decisions.
- DNA Methylation Analysis With Carrier DNA and Unique-End Tagging
Carrier DNA and unique-end transposome tagging cut sample loss during methylation analysis, enabling single-cell sequencing with uniform coverage.
- Dual-Index Nucleic Acid Fragments for Accurate Read Assignment
A dual-indexing scheme assigns diverse index pairs to each nucleic acid fragment, reducing index hopping and read misassignment in low-plexity sequencing.