Oxidoreductases TRIZ Cases

A recombinant host cell converts glucose to xylitol using NAD+-specific D-arabitol 4-oxidoreductase and NADPH-specific xylitol dehydrogenase.

Specific AlkB mutations at positions V129 and T136 reduce over-oxidation to dicarboxylic acids, increasing HLAME yield while maintaining green biocatalysis.

Engineered yeast cells replace expensive chemical catalysts to produce very long chain fatty acids via modular biological pathways.

Enzymatic oxidation using specific cytochrome P450 monooxygenases replaces costly classical isolation steps from natural oils.

Cupriavidus necator expresses cis-aconitate decarboxylase to boost itaconic acid titers while avoiding protein aggregation from heterologous enzymes.

A recombinant polypeptide catalyzes substrate prenylation to produce cannabinoids in a cell-free environment.

Coexpressing Campanula flavonoid 3′,5′-hydroxylase and Clitoria anthocyanin glucosyltransferase bypasses polyacylation to achieve true blue hues.

Endpoint TaqMan PCR assays eliminate denaturing steps to resolve DNA quality sensitivity bottlenecks in large-scale zygosity analysis.

Short interfering nucleic acids silence dopamine-beta-hydroxylase to lower intraocular pressure and slow optic neuropathy progression.

The 24DT21 gene enables safe 2,4-D application by metabolizing the herbicide via hydroxylation and conjugation, preventing dicot crop damage.

Alpha-glucosidase and glucose oxidase create firm centers and elastic surfaces, resolving texture uniformity trade-offs.

Wild-type bacterial enzymes replace transition metal complexes in reductive amination, eliminating waste while maintaining high enantiomeric excess.

Engineered oleaginous yeast produces desaturated fatty alcohols through heterologous enzyme expression, lowering production costs and environmental impact.

Enhanced fructose 6-phosphate epimerase and phosphatase convert substrates to tagatose, resolving costly separation and low yield bottlenecks.

Sorbitol dehydrogenase gene modulation alters maize seed size, number, and sugar profiles through targeted genetic expression changes.

Deleting the tyrB gene eliminates byproduct pathways, resolving the trade-off between high purity and low production cost.

Next-generation sequencing identifies the uncultivable symbiont's gene cluster, allowing ET-743 synthesis in host cells without natural source yields.

Phosphoketolase converts D-fructose to acetyl phosphate without ATP expenditure, increasing acetyl-CoA precursor yield.

Threonine residue substitution at position 149 stabilizes laccase protein structure, preventing aggregation and boosting recombinant yield in bacterial hosts.

Site-directed mutagenesis at positions 95, 108, 172, and 303 boosts enzyme activity 33-fold, reducing reaction time from 20 hours to 120 minutes.

Fermentation coupled with solvent extraction isolates methacrylate esters for subsequent transesterification into methyl methacrylate.

Deleting competing dehydrogenase genes reduces fructose by-product accumulation, raising the L-sorbose transformation rate to 96.12%.

Directed evolution of the POX gene modifies metabolic pathways to suppress short-chain dibasic acid formation during fermentation.

Introducing sulfhydryl oxidase into E. coli cytoplasm resolves the contradiction between low production costs and reliable disulfide bond formation.

Mutating residues A166, V376, and T196 in L-glutamate dehydrogenase enables high substrate concentration biocatalysis, resolving low efficiency bottlenecks.

Cell-free systems perform enzymatic hydroxylation and glycosylation to replicate natural collagen structures while maintaining industrial scalability.

Site-directed mutations in the substrate binding site of NMAADH improve catalytic activity and specificity for synthesizing N-alkyl amino acids.

Engineered alcohol dehydrogenase mutants catalyze asymmetric reduction of prochiral ketones to produce optically pure chiral diaryl alcohols.

Mutated HPPD polypeptides alter enzyme binding to resist herbicide inhibition, maintaining catalytic activity while conferring tolerance.

Mutated Diktyoglomus xylose isomerases maintain stability in crude hydrolysates, enabling efficient conversion without purification.

Novel 7β-HSDH enzyme catalyzes stereospecific reduction of dehydrocholic acid to produce ursodeoxycholic acid with high selectivity.

Amino acid substitutions enhance preservation stability in modified cholesterol oxidase, resolving surfactant interference for clinical diagnostics.

Engineered ketoreductases resolve cofactor regeneration and stability contradictions, enabling industrial chiral alcohol production.

Replacing chemical reducing agents with enzymatic systems to eliminate harmful residues and simplify purification.

Engineered acetogens produce hydrocarbons from gaseous substrates using alternative metabolic pathways, reducing ATP consumption and increasing yield.

Deleting the panD gene eliminates pantothenic acid accumulation, simplifying purification and lowering production costs.

Recombinant microbes express carboxylic acid reductase to synthesize C8-C24 fatty alcohols, addressing the need for sustainable biofuel feedstocks.

Eukaryotic cell lysate with membrane vesicles enables functional Cytochrome P450 synthesis, overcoming low yields in bacterial expression.

Cloned delta-8 desaturase genes enable PUFA production pathways that bypass GLA and SDA formation, resolving productivity versus harmful factor contradictions.

Multi-enzyme formulation converts raw plant biomass into spinnable textile fibers without harsh chemical pre-treatment.

Engineered yeast strains convert glucose to vanillin using shikimate pathway intermediates, addressing low yield and high cost of natural production.

Ketoreductase enzymes bioconvert hop isoalpha acids into light-stable dihydro compounds, eliminating hazardous sodium borohydride reagents.

Segmenting fungal biosynthetic pathways into modular gene components enables controlled production of antimicrobial sesquiterpenoids in heterologous hosts.

Mutated ABA 8'-hydroxylase enzymes modify seed dormancy levels, preventing pre-harvest sprouting in wheat and barley crops.

Transgenic microorganisms express chalcone isomerase and enoate reductase to convert flavanone glycosides into dihydrochalcones.