Peptide Preparation Methods TRIZ Cases
- Antibody Purification Air Overlay Depth Filtration
Air overlay and low flux depth filtration prevent disulfide bond reduction in antibodies.
- Hydrophobic Protein Separation via Mixed Mode Chromatography
Segmented purification with non-ionic detergent washes removes co-eluting host cell proteins during Protein A capture to improve biotherapeutic purity.
- rAAV Purification via Anion Exchange and Zonal Ultracentrifugation
Anion exchange chromatography removes charged impurities from adeno-associated virus particles before zonal ultracentrifugation refines density-based contaminants.
- Caspofungin Preparation via Borane Reduction and Leaving Group Substitution
A caspofungin preparation method uses borane complex reduction and strong leaving group substitution to synthesize the target compound.
- L-Amino Acid Production via c1795 Gene Modification
Reducing c1795 protein activity increases PAJ_1175, PAJ_1174, and PAJ_1173 expression to boost L-amino acid yields.
- Hydantoin Peptide Synthesis via Orthogonal Protecting Groups
Pre-synthesized hydantoin blocks with orthogonal protection minimize by-product formation during solid-phase peptide assembly.
- Organic Aerogel Maleimide Vinyl Polymer Strength
Maleimide and vinyl compounds polymerize into organic aerogels that resolve flexibility versus strength trade-offs.
- Peptide Stapling via Phase-Transfer Tetrazine Chemistry
Phase-transfer catalysis enables efficient staple incorporation into reactive peptides without decomposition, resolving stability trade-offs.
- Radiohalogenated Prosthetic Agents for Site-Specific Protein Labeling
Novel radiohalogenated prosthetic agents form stable thioether bonds with cysteine residues on proteins.
- Site-Specific Serine ADP-Ribosylation via Phosphorylation Protection
Phosphorylating specific serines protects them during enzymatic reactions, enabling pure site-specific ADP-ribosylation for antibody generation.
- Photoactive Peptide Coupling Formulation for Microarray Synthesis
Light-exposed tetrazole-thione reagents drive high-efficiency peptide coupling on microarrays, resolving low yield bottlenecks in parallel synthesis.
- Salty Taste Enhancing Peptides from Dry-Cured Ham
Peptides extracted from dry-cured ham boost salty taste perception, resolving the contradiction between flavor enhancement and excessive sodium intake.
- Solid Phase Peptide Synthesis Lysine Deprotection
Vacuum drying removes residual DMF before TFA treatment, ensuring complete lysine deprotection and high peptide purity during scale-up.
- Polyisobutene Anchoring for Liquid Phase Peptide Synthesis
Polyisobutene anchors solubilize amino acids in apolar solvents to enable liquid phase peptide synthesis.
- Recombinant Pichia pastoris for active pediocin PA-1 production
A recombinant Pichia pastoris strain secretes active pediocin PA-1 into the culture medium.
- Polysorbate 20 Protein Precipitation Prevention
Polysorbate 20 prevents precipitation during affinity chromatography, ensuring high yield and purity.
- Cyclic Carbamate Ligation for Glycoprotein Synthesis
Cyclic carbamates resolve chemoselectivity trade-offs by forming non-toxic glycosylurea linkages without coupling reagents.
- Ligand-Functionalized Substrates for Virus Purification
Grafted electrophilic and ionic groups on porous substrates enhance binding selectivity, reducing pressure drops during high-throughput viral purification.
- Phycocyanin Purification via Aqueous Two-Phase Separation
A method partitions phycocyanin into a carbohydrate phase using aqueous two-phase separation.
- Peptide Synthesis Additives Suppress Epimerization
Specific additives suppress epimerization and double insertion during carbodiimide peptide synthesis to improve purity.
- Cyclohexapeptide Isolation via Resin Adsorption and Crystallization
Resin adsorption concentrates cyclohexapeptides to avoid intermediate steps that cause racemization and yield loss during purification.