Transferases TRIZ Cases

Altered acetolactate synthase enzymes in sorghum resist herbicide inhibition through specific amino acid substitutions.

A truncated hST6Gal-I variant enables quantitative sialylation control on glycoproteins.

Exogenous cyanobacterial polypeptides bypass inhibited endogenous pathways to restore plastoquinone-9 levels and herbicide tolerance.

Site-directed mutations in recombinant ORF2 enzymes replace complex chemical synthesis, lowering production costs while increasing cannabinoid yield.

Codon-optimized D-lactate dehydrogenase maintains activity at 50-60°C, resolving heat stability limits in industrial fermentation.

Mutant DNA polymerases with specific amino acid substitutions enhance nucleic acid extension rates and reverse transcription efficiency.

Chimeric enzyme structures overcome the trade-off between thermostability and strand displacement, enabling reliable long sequence amplification in PCR.

Engineered transaminase polypeptides resolve enzyme instability and narrow substrate recognition by introducing specific amino acid residue modifications.

An R47C substitution in the RNA polymerase beta prime subunit decreases plasmid replication to resolve stability trade-offs.

A site-directed integration method uses DNA enzymes to target donor vectors into pre-defined genomic locations within host cells.

A Candida viswanathii gene editing system uses linear fragments with homology arms and Cas9 cassettes for precise chromosomal modification.

Deleting the PFK-2 gene reduces glycolytic flux to resolve trade-offs between cell growth and metabolite yield, achieving 2 g/L production.

A spermidine synthase variant with an alanine-to-valine substitution boosts L-valine production in Corynebacterium glutamicum strains.

SsrA-tagged enzymes direct metabolic flux to boost L-amino acid yields while maintaining bacterial growth.

Heterologous expression of ET-743 biosynthetic gene clusters overcomes uncultivable symbiont constraints for scalable production.

Engineered DNA polymerase mutations sustain high mutation rates during culture, eliminating iterative cloning steps required for directed evolution.

Engineered yeast converts pyruvate to 2,3-butanediol using heterologous enzymes and NADH oxidase, resolving safety risks from pathogenic bacteria.

Specific amino acid substitutions in the N-terminal domain and catalytic core improve amplification yield and sensitivity while maintaining thermal stability.

Segmented modified oligonucleotides reduce DMPK expression and alleviate myotonia symptoms by preferentially degrading CUGexp RNA through RNase H cleavage.

Engineering Escherichia coli with propionyl-CoA carboxylase and methylmalonyl-CoA mutase produces branched-chain fatty acids with specific branch positions.

A T7 expression system integrates a plasmid stabilization system to maintain vector integrity during recombinant protein production.

Autonomous P1 plasmid replication eliminates manual transformation steps, accelerating affinity maturation and improving scalability.

The AuOS gene replaces complex chemical synthesis with enzymatic cyclization to resolve structural accuracy and process complexity trade-offs.

Genetically modified bacterial host cells synthesize human milk oligosaccharides using engineered metabolic pathways.

Site-directed mutagenesis of Squalene Hopene Cyclase improves stereoisomer purity and yield during homofarnesol conversion to ambroxan.

Terpene synthases eliminate phosphorus molecules from alkyl monoesters, replacing petroleum cracking with sustainable bioproduction.

Enzymatic polypeptide scaffolds organize heterologous enzymes via cohesin-dockerin interactions to form structured metabolic pathways.

Introducing the ynfM gene into production strains resolves accumulation bottlenecks by enabling efficient extracellular transport of glutaric acid.

Novel beta-alanine/alpha-ketoglutarate aminotransferase sequences increase malonyl semialdehyde conversion rates.

Protease mixtures release amino acids to boost ethanol yield while lowering nitrogen supplementation needs.

Enzymatic cross-linking replaces hazardous chemicals to improve strength and sustainability in foundry moulds.

Cellular energy inhibitor formulations disrupt glycolysis to eliminate infected cells and prevent cytokine storms.

Mutant alleles modify enzyme activity to lower raffinose and stachyose levels, resolving the trade-off between nutritional quality and germination rates.

Deleting the 3′→5′ exonuclease domain from Bst DNA polymerase improves heat resistance and storage stability while maintaining strand displacement activity.

Overexpressing CODH and acetyl CoA reductase while downregulating acetate kinase shifts metabolic flux from acetate formation to improve ethanol yield.

Deleting the J2R gene creates a thymidine kinase-deficient vaccinia virus that sensitizes cancer cells to ganciclovir while sparing normal tissue.

Codon-adapted Δ5-elongase sequences boost polyunsaturated fatty acid yields in transgenic Brassica, bypassing complex strain mutation processes.

Overexpressing cysG, cysJ, and cysI genes in bacteria improves thiosulfate utilization efficiency for L-cysteine production.

Physical attachment of recombinant transaminases to resin stabilizes enzymes in 90% organic solvents, enabling catalyst reuse and high enantiomeric excess.

Targeted FUT8 gene inactivation via zinc finger cleavage domains eliminates laborious screening while boosting monoclonal antibody therapeutic efficacy.

Genetic modification relieves catabolite repression, allowing efficient co-metabolism of mixed sugars from lignocellulosic biomass.

Tandem-expressed siRNAs target Bruton tyrosine kinase mRNA to suppress protein levels in chronic lymphocytic leukemia cells.

A mutant Taq DNA polymerase with specific amino acid substitutions at positions 783 or 784 enhances template discrimination activity.