Transferases TRIZ Cases

Specific T7 polymerase substitutions improve co-transcriptional capping and reduce dsRNA contamination in RNA transcription.

Knock-in engineering redirects metabolism for stable fermentation and higher product titers.

This case uses CaMK4 inhibitors to reduce skin thickness, scaling, and epidermal inflammation in psoriasis models.

Targeted residue changes stabilize transaminases for stereoselective chiral amine synthesis.

Targeted amino acid substitutions tune glucosyltransferases for more alpha-glucan and less leucrose byproduct.

Site-directed transaminase variants convert prochiral ketones into chiral tryptamine derivatives with over 90% enantiomeric excess.

LfCPT1, LfTPS1, and CYP71D616 form a modular pathway for scalable serrulatane diterpenoid production and non-natural analog access.

Seven maternal plasma biomarkers track protein abundance changes to distinguish true labor and reduce unnecessary admissions.

Engineered Corynebacterium stabilizes PAPS production for sulfated polysaccharide synthesis.

A two-stage process uses stable imino acid reductases to convert 1-position unsaturated cyclic amino acids into high-purity L-forms.

A soluble SB transposase variant supports protein delivery with controlled transposition.

Targeted TdT substitutions improve 3′-O-blocked nucleotide incorporation for stable, template-free polynucleotide synthesis.

Pseudomonas putida ω-transaminase expands substrate scope and improves conversion to (R)-3-amino-1-butanol.

A heterologous fungal NHMT enzyme uses SAM to modify N-terminal histidine, improving protein stability, activity, and solubility.

This case uses large serine recombinases with attP and attB sites to improve genomic precision while managing system complexity.

CD68+p-PYK2+YAP1+ macrophages provide a pancreatic cancer index, while PYK2 and PD-1 inhibition suppresses tumor growth.

This case engineers microorganisms with Mdh/Mox and Das/transketolase to convert methanol into growth and carbon-carbon compounds.

RNA sequencing and machine learning scale tumor antigen discovery for adoptive T-cell therapy.

Specific UDP-glycosyltransferases add glycosyl groups at C20, C6, and C3, enabling controlled synthesis of diverse ginsenosides.

Specific A3/A10 mutations help one enzyme reverse-transcribe RNA and amplify cDNA under shared reaction conditions.

Engineered transporters boost melatonin and 5HTP output while limiting cellular toxicity.

This case uses an A303T HSK mutation to balance downy mildew resistance, powdery mildew tolerance, and squash productivity.

This case uses a codon-optimized recombinant vector to express replicase that recognizes RdRp sites for efficient RNA amplification.

Specific amino acid mutations improve reverse-transcriptase thermostability, transcription, and template switching in low-volume reactions.

This case uses mutated UstD with PLP to convert aldehydes and amino acids in one step, improving selectivity and turnover.

To address complex ACCase regulation, CTI expression tunes α-CT activity and alters fatty acid, triacylglycerol, and seed oil production.

Targeted TdT substitutions support efficient modified-nucleotide incorporation, improving quality and reducing synthesis turnaround time.

This case addresses transient RNA interference with integrated artificial microRNAs that sustain gene suppression across mammalian cell divisions.

This case uses AcbB and GtaB overexpression plus Cgt and carotenoid pathway reduction to improve acarbose formation.

This case uses pre-assembled plasmid and viral vectors to increase endogenous TERT mRNA production and protein bioavailability.

Targeted amino acid substitutions reduce dsRNA by-products while preserving transcriptional activity, yield, and RNA integrity.

This case pairs engineered L-threonine transaldolases with carboxylic acid reductases to stabilize aldehydes during aerobic fermentation.

Engineered acetate kinases catalyze ATP generation, simplifying production of nucleotide compounds that inhibit HIV reverse transcriptase.