Transferases TRIZ Cases
- Modified Lactose Permease for Oligosaccharide Production
Modifying lactose permease amino acids increases 2'-fucosyllactose yield by enhancing substrate uptake efficiency.
- Recombinant Microorganism Producing High-Molecular-Weight Polylactate from Sucrose
Recombinant microorganisms convert sucrose into high-molecular-weight polylactate, eliminating organic solvents and chain coupling agents.
- Thermostable Polymerase Variants for Nanopore Sequencing
Engineered polymerase variants enhance thermostability and sequencing efficiency through directed evolution.
- Biosynthetic Heparin Production via Chemoenzymatic Sulfation
Chemoenzymatic sulfation of heparosan intermediates produces biosynthetic heparin that meets USP bioequivalence standards for porcine heparin.
- Rubber Particle Protein Complex Enhances Natural Rubber Synthesis
CPT, NgBR, and REF proteins bind to rubber particles to boost synthesis activity without damaging trees via chemical inducers.
- JAK1 Selective Inhibitors via Cysteine 817 Covalent Bonding
Electrophilic warheads form covalent bonds with cysteine 817 to resolve the trade-off between therapeutic efficacy and off-target kinase inhibition.
- Recombinant Yeast Synthesizing Andrographolide Intermediate via CYP450 Enzymes
Heterologous CYP71A8 and CYP71D10 expression in recombinant yeast enables de novo synthesis of the key andrographolide intermediate.
- Engineered tRNASec for SECIS-Independent Selenocysteine Incorporation
Engineered tRNASec allows selenocysteine incorporation without a SECIS element, resolving manufacturing complexity and improving production efficiency.
- LovD Mutants for Simvastatin Fermentation
Engineered LovD variants catalyze direct simvastatin synthesis via acyl thioester transfer, eliminating lovastatin purification steps.
- Biomimetic Condensate Scaffold for Enzyme Concentration
Biomimetic condensates concentrate enzymes and substrates via liquid-liquid phase separation to enhance catalytic efficiency.