Vector-Based Foreign Material Introduction TRIZ Cases
- Soybean Variety 5PTNN70 Trait Combination for Stable Yield
Preselected parental lines and feedback-based progeny selection combine disease and stress resistance with stable soybean yields.
- Soybean Variety 5PENE00 for Multi-Disease and Abiotic-Stress Tolerance
5PENE00 combines native and transgenic traits to address long breeding cycles with multi-disease resistance and drought and heat tolerance.
- Cytokinin-Induced Plant Shoot Regeneration Using CHK Histidine Kinases
Limited plant cell regeneration is addressed by expressing CHK2, CHK3, or CHK4 in cytokinin media to promote shoot formation.
- Plant Promoter Control Elements for Tissue-Specific Gene Expression
This case uses plant promoter control elements to target transcription by tissue and developmental stage, reducing harmful misexpression.
- Female Doublesex Gene Drive Targeting Ultra-Conserved Exon 5 Splicing
This case targets the female-specific doublesex exon 5 boundary to suppress reproduction while limiting resistance variants.
- Plant Regulatory Sequences: Intronic Enhancers for Stable Transgene Expression
Modular promoters, introns, and terminators support stacked plant traits while improving transgene expression stability.
- Chimeric RNA Meristem Delivery for Tissue-Culture-Free Plant Genome Editing
Meristem-targeting RNA carries editing cargo through the phloem, bypassing tissue culture for controlled plant genome modification.
- HEK293 Suspension Platform Optimization for High-Titer rAAV Production
This case uses segmented plasmids and tuned molar ratios in HEK293 suspension cells to achieve high-titer rAAV production.
- Polyploid Maize Breeding With Clonal Gametes for Uniform Seeds
MiMe-based clonal gamete formation enables maize crosses that produce uniform polyploid seeds with multiple haplotypes.
- Bacillus Subtilis Virus Vector Plasmid Production With Low Endotoxin
This case uses Bacillus subtilis replication and controlled amplification to produce virus vector plasmids with high CCC purity.
- Plant Protein Expression Vectors with Composite Promoters and Terminators
This case combines viral promoter fragments and repeated terminators to increase plant protein expression for mass production.
- Humanized IL-15 Animals with Endogenous Locus Regulation
Endogenous IL-15 regulation supports human protein expression without immune pathologies.
- Covalently Closed Parvoviral Vectors for Direct Transgene Expression
ccePV vectors bypass second-strand synthesis for direct transgene expression.
- Gene-Edited Citrus Plants for Huanglongbing Resistance
Gene-edited citrus plants target bacterial effectors to resist HLB.
- Polyploid Maize Breeding Using MiMe Clonal Gametes
MiMe clonal gametes enable uniform polyploid maize seed production.
- Circular Plant Vectors Using eccDNA for Stable Gene Amplification
This case uses tethered circular vectors and replicon proteins to amplify plant genes without chromosomal integration.
- Self-Replicating Recombinant RNA for Precise Plant Phenotype Modification
Endornaviral RdRP recognition sequences enable cargo RNA replication and expression for plant stress, pest, and pathogen resistance.