Vectors TRIZ Cases
- AAV Capsid Peptide Inserts for CNS and Muscle Tropism
Targeted peptide inserts in AAV capsids improve CNS and muscle transduction while helping evade pre-existing neutralizing antibodies.
- Liver-Specific Cis-Regulatory Elements for High-Expression Gene Therapy
Synthetic liver-specific enhancer elements boost hepatic gene expression in compact vectors while minimizing off-target expression.
- Mutant rtTA Transactivators for Low-Leakage Tet-On Gene Control
Four rtTA mutations improve tetracycline sensitivity and suppress Tet-On leakiness, enabling tighter and less toxic transgene expression.
- Plasmid Addiction via Succinate Pathway for Antibiotic-Free Retention
A succinate-pathway balanced lethal strategy keeps expression plasmids in daughter cells without antibiotics, lowering fermentation cost.
- Corynebacterium Promoter Engineering for Glycine and Glutamate Production
Modified promoter nucleotides boost HisE and HisG expression in Corynebacterium, raising glycine output while preserving glutamic acid productivity.
- Multi-Input miRNA Sensing Circuits for Cell-Type Classification
Constitutive ERN circuits classify cells from miRNA profiles while reducing toxicity and enabling rapid multi-output gene expression.
- CG-Rich β-Actin/CMV Promoter for Stable Recombinant Protein Expression
A CG-rich β-actin fragment placed upstream of CMV helps prevent promoter silencing and boosts recombinant antibody yield in CHO cells.
- UCP1-Regulated VGLL4 Construct for Brown Fat Expansion
A UCP1 enhancer-promoter drives VGLL4 expression in brown fat and liver cells to increase BAT, reduce WAT, and improve metabolic health.
- Capsid-Modified rAAV Vectors for MTC Cell-Specific Gene Delivery
Modified rAAV capsids and a truncated calcitonin promoter improve MTC cell transduction and targeted therapeutic gene expression.
- SRF-UCOE Chromatin Element for Stable Long-Term Transgene Expression
A compact SRF-UCOE keeps chromatin open to resist silencing, methylation, and position effects for durable transgene expression.
- gp120-Binding HIV-1 Antibodies for Broad Neutralization
Engineered gp120-binding sites target conserved HIV-1 epitopes to improve neutralization breadth, potency, and resistance to viral escape.
- AAV Capsid Variants for Antibody-Evasive CNS Gene Delivery
Specific AAV capsid amino acid changes improve CNS and liver tropism while helping vectors evade pre-existing antibody neutralization.
- Self-Replicating RNA Replicons for High Expression, Low Immunogenicity
Viral replicase and optimized UTR design boost self-replicating RNA expression while lowering immunogenicity and dose needs.
- Renal Artery Viral Vector Delivery for Targeted Glomerular Gene Transfer
Direct renal artery catheter infusion improves viral vector delivery to glomeruli and podocytes while limiting systemic spread.
- Dual-Vector Insulator Screening for Silencer-Free Genome Mapping
A dual-vector MAI-seq layout separates insulators from silencers, enabling accurate genome-wide screening across species.
- Globin Expression Cassettes With Insulators for Safer Gene Therapy
Enhancer-blocking insulators and modified globin control regions enable erythroid-specific expression while lowering insertional oncogenesis risk.
- Quorum-Sensing Auto-Induction for Balancing E. Coli Growth and Expression
A LuxI/LuxR promoter system auto-induces target gene expression in engineered E. coli, balancing growth and production without toxic inducers.
- Oncolytic Adenoviral Vector for Tumor-Selective Immune Activation
Hypoxia- and inflammation-responsive adenoviral replication improves tumor selectivity while CD40L and 4-1BBL boost antitumor immunity.
- VA RNA Promoter Design for Controlled rAAV Packaging Cell Lines
Inducible VA RNA transcription helps rAAV packaging cell lines avoid toxic rep/helper expression while improving production uniformity.
- Multi-circRNA AAV Constructs for High Tissue Expression
AAV delivery of tandem circRNA constructs with intronic elements and IRES boosts multi-circRNA expression across heart, liver, and muscle.
- Engineered M13 Phage Capture of SARS-CoV-2 at Low Viral Loads
Engineered M13 phages bind Spike S1 to capture and concentrate SARS-CoV-2 from samples, enabling sensitive detection at low viral loads.
- Self-Circularized RNA Structure for Longer Protein Expression
Group I self-splicing introns and stable end structures circularize RNA to resist exonuclease decay and extend protein expression.
- Gene-Silencing Composition for Plant Salt Stress Tolerance
Targeted inhibition of salt-response nucleotide sequences improves plant or algae survival and growth under high salinity.
- Codon 3-5 Sequence Tuning for Precise Protein Translation Control
Targeted nucleotide changes at codons 3, 4, and 5 tune protein abundance without changing amino acid sequence or using complex expression methods.
- Renal Artery Catheter Delivery of Viral Vectors to Glomeruli
Catheter infusion through the renal artery bypasses glomerular permselectivity to improve podocyte gene delivery with kidney-localized expression.