Vectors TRIZ Cases
- AAV VP1 Capsid Mutations for Higher Ocular Transduction
Targeted VP1 amino acid substitutions in AAV capsids improve intracellular trafficking and ocular cell transduction while preserving a human AAV backbone.
- Granulosa-Like Cell Induction via NR5A1 and RUNX Overexpression
NR5A1 and RUNX overexpression drives pluripotent stem cells into granulosa-like cells in 5-7 days with robust markers and hormonal function.
- Alphaherpesvirus LAP Promoters for Long-Term AAV Transgene Expression
Small alphaherpesvirus LAP promoters help AAV vectors avoid transcriptional repression and sustain chronic transgene expression.
- AAV Capsid Peptide Insertions for Eye-Targeted Gene Delivery
Peptide insertions in AAV capsid variable regions IV or VIII improve eye-cell targeting and transduction for ocular gene delivery.
- Targeted RNA Circularization for Cell-Specific Therapeutic Expression
Self-splicing introns and homology arms circularize RNA only in target cells, improving expression stability while avoiding genomic integration.
- mRNA 3' UTR U-Rich Sequence for Higher Translation Efficiency
A U-rich 3' UTR placed after the stop codon boosts mRNA stability and translation, enabling lower therapeutic doses and cost.
- Modified Poly(A) Structure for Stable mRNA Translation
Segmented poly(A) blocks with non-A spacers stabilize mRNA tail length and improve translation consistency for RNA therapeutics.
- Modified Pre-crRNA for Efficient and Allele-Specific Cas3 Editing
Localized nucleotide modification in pre-crRNA boosts Cas3 editing efficiency and supports allele-specific editing with clearer pattern analysis.
- Macrophage Cholesterol Catabolism Using Bacterial Sterol Enzymes
Bacterial sterol-degrading enzymes let modified macrophages break down cholesterol, addressing foam-cell buildup linked to atherosclerosis.
- Inducible Split Recombinases for Low-Toxicity Gene Expression Control
Inactive recombinase halves dimerize only after induction, reducing cell toxicity while enabling precise gene expression control.
- Artificial Nucleic Acid Molecules for Stable High-Expression RNA
TOP-derived 5′UTR elements and histone stem-loops help RNA resist RNase degradation while boosting translation and protein expression.
- Genetically Engineered Mouse Models for Human-Like Multiple Myeloma
Conditionally activated MM transgenes in immunocompetent mice capture disease progression and tumor-immune interactions for drug screening.
- Asymmetric AAV ITR Platform for Higher Expression and Lower Genotoxicity
An asymmetric AAV ITR design boosts DNA packaging and transgene expression while reducing host genome integration and genotoxicity.
- Plant Promoter Sequences for Stable Constitutive Gene Expression
Specific plant promoter sequences and GATCTG motif editing improve constitutive gene expression stability and reduce variation in transgenic plants.
- AAV Podocyte-Targeted Gene Therapy for Monogenic Nephrotic Syndrome
Podocyte-specific AAV vectors use NPHS1 or NPHS2 promoters to restore functional gene expression and reverse monogenic nephrotic syndrome.
- CpG-Reduced AAV Vector Composition for Low-Immunogenic Payload Delivery
Reduced-CpG AAV vectors use methylation, CAG promoter control, and WPRE to sustain payload expression while limiting immune activation.
- MicroRNA-Targeted Polynucleotides for Cell-Selective Expression
MicroRNA target sites in therapeutic polynucleotides enable cell-selective expression, reducing off-target effects in mRNA and gene editing.
- Synthetic AAV Capsids for Liver-Specific Gene Transfer
Engineered AAV capsids use DNA shuffling and in vivo screening to boost liver-specific transduction while limiting off-target gene transfer.
- Granulosa-Like Cell Differentiation Using NR5A1 and RUNX Overexpression
NR5A1 and RUNX overexpression drives fast, serum-free iPSC differentiation into granulosa-like cells with proper markers and hormonal function.
- Linear DNA with NLS and End Adaptors for Nuclear Delivery
End-adapted linear DNA with an appended NLS improves nuclear entry in non-dividing cells while resisting exonuclease digestion.