Vectors TRIZ Cases
- Self-Complementary AAV Vector Bypassing Second-Strand Synthesis
Self-complementary AAV bypasses cellular second-strand synthesis to increase β-sarcoglycan expression in LGMD2E.
- Podocyte-Specific Hybrid Promoters for Large Gene Expression
A compact enhancer–core promoter hybrid enables selective expression of larger genes in podocytes within AAV size constraints.
- Chimeric TDP-43 Proteins for Splicing Recovery Without Aggregation
The TDP-43 RNA-binding domain is fused to RAVER1 to restore splicing repression while limiting cytoplasmic aggregation in motor neuron models.
- Engineered T7 RNA Polymerase Fusion for Capped RNA Transcripts
T7 RNA polymerase is fused with NP868R to add 5′ caps during transcription, enabling functional capped RNA production in eukaryotic cells.
- Tetracycline-Inducible Rep-Cap Plasmids for Efficient rAAV Production
Inducible viral-gene expression and helper functions address low rAAV output, increasing production by 1.5–2-fold.
- Short Hepatocyte-Specific Promoters for Selective Gene Expression
By isolating essential promoter elements, 123–252 bp nucleic acids retain activity while shortening expression cassettes for hepatocytic cells.
- Chinese Hamster Eno1 Promoter for Higher Protein Production
Conventional promoters can limit cultured-cell productivity; Chinese hamster Eno1 promoters raise foreign protein expression while retaining mammalian cell quality.
- Modified-Intron UGT1A1 Constructs for AAV Hyperbilirubinemia Therapy
Modified HBB2 introns and codon-optimized UGT1A1 in AAV vectors target liver cells to lower bilirubin and reduce immune response.
- Recombinant Bagworm Silk Through Transgenic Silkworm Production
Modified bagworm Fib H lets transgenic silkworms produce hybrid silk at scale, avoiding contaminated natural nests and costly cleanup.
- Liver-Specific Regulatory Elements for Compact Gene Therapy Vectors
Compact enhancer elements linked to minimal promoters preserve liver-specific expression while leaving vector space for large effector genes.
- Synthetic AAV Capsids for Liver-Specific Gene Transfer
DNA shuffling and mouse-liver screening select synthetic AAV capsids for liver gene transfer while limiting off-target transduction.