Vectors TRIZ Cases
- Segmented PolyA Polynucleotide for Stable mRNA Plasmid Fermentation
Linker-separated PolyA segments stabilize plasmids in E. coli, helping control mRNA tail length and improve production consistency.
- Modified Muscle-Specific Promoters for Full-Genome AAV Packaging
This case modifies muscle-specific promoters to reduce hairpin structures, improving full-genome AAV packaging and simplifying purification.
- Lentiviral Vectors Disrupting Internal ORFs for Safety and Capacity
Disrupting internal open reading frames in lentiviral vectors improves safety while deleting Gag-p17 sequences increases transgene capacity.
- Expressed Barcode Libraries for Cell Lineage and Transcriptional State Profiling
Integrated nucleic acid constructs merge lineage tracing and reporter genes to de-convolute non-genetic resistance mechanisms in cancer cells.
- Cyclized Cytokine Production via Split Intein Trans-Splicing
Split intein trans-splicing connects termini of linear mutants, achieving high cyclization efficiency while retaining biological properties.
- Selective Renal Cell Differentiation via Defined Culture Medium
Defined culture medium with TGFβ, Wnt, and phosphodiesterase inhibitors produces renal collecting duct principal cells.
- Automated Viral Vector Production Using Derepressible Promoters
Derepressible promoters in enclosed systems boost vector yield and consistency while cutting production time.
- Induced Astrocyte Generation via Doxycycline-Inducible Transcription Factors
A doxycycline-inducible protocol overexpresses Nfib, Sox9, and Nfia to rapidly generate induced astrocytes from human pluripotent stem cells.