187A5 Gene and Protein Markers for Dopaminergic Neuron Progenitor Cell Isolation

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Solution Overview

Problem

Current methods for treating Parkinson's disease, such as transplanting dopaminergic neuron progenitor cells, face challenges like ethical issues, immunologic rejection, low survival rates, and the need for complex procedures to isolate specific dopaminergic neurons from mixed cell populations, which complicates neural network formation and treatment efficacy.

Innovation Solution

A probe or primer that hybridizes to a specific polynucleotide sequence of the 187A5 gene, and an antibody that recognizes the 187A5 protein, are used to detect and isolate dopaminergic neuron progenitor cells, allowing for the selection of pure dopaminergic neuron progenitor cells for transplantation, enhancing treatment safety and efficacy by ensuring correct network formation and prolonged therapeutic effect.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If dopaminergic neuron progenitor cells are transplanted to treat Parkinson's disease, then the therapeutic effect is improved, but the risk of immunologic rejection and low survival rate increases

Engineering Contradiction:

Improvetherapeutic effectVSAvoidimmunologic rejection

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent uses the patient's own induced pluripotent stem cells (iPSCs) to generate dopaminergic neuron progenitor cells for transplantation. This self-service approach ensures MHC matching between donor and recipient, completely eliminating immunologic rejection risk while maintaining therapeutic efficacy

Inventive Principle:

Principle #25Self-service

2Manufacturing precision

If dopaminergic neuron progenitor cells are isolated from mixed cell populations, then the purity of transplant cells is improved, but the complexity of the procedure increases

Engineering Contradiction:

Improvecell purityVSAvoidisolation procedure

Core Design Contradiction:

Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent employs fluorescent markers (such as GFP) that are genetically expressed in dopaminergic neuron progenitor cells, allowing these cells to emit fluorescent signals. This enables rapid identification and isolation of pure dopaminergic neuron progenitor cells from mixed cell populations using flow cytometry or fluorescence-activated cell sorting (FACS), dramatically simplifying the isolation procedure while ensuring high cell purity

Inventive Principle:

Principle #32Color changes

Solution Approach 2:

The patent replaces complex mechanical manual isolation methods with fluorescence-based detection and automated cell sorting systems. This substitution enables precise identification and separation of dopaminergic neuron progenitor cells based on their fluorescent markers, achieving high purity without manual intervention

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

3Quantity of substance

If fetal tissue is used for transplantation, then the supply of dopaminergic neurons is improved, but ethical issues and infectious contamination risk increase

Engineering Contradiction:

Improvecell supplyVSAvoidinfectious contamination

Core Design Contradiction:

Quantity of substanceVSObject-affected harmful factors

Solution Approach 1:

The patent uses induced pluripotent stem cells (iPSCs) derived from the patient's own somatic cells (such as skin fibroblasts or blood cells) as a renewable source for generating dopaminergic neuron progenitor cells. This disposable-like approach allows unlimited expansion of patient-specific cells in vitro, providing sufficient cell supply without ethical concerns or infectious contamination risks associated with fetal tissue

Inventive Principle:

Principle #27Cheap short-living objects (Disposable)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The use of the 187A5 gene and protein markers enables the precise detection and isolation of dopaminergic neuron progenitor cells, improving the purity and survival rate of transplanted cells, thereby enhancing the effectiveness of Parkinson's disease treatment by promoting accurate neural network formation and prolonged therapeutic benefits.

Implementation Method 1

A probe or primer that hybridizes to a specific polynucleotide sequence of the 187A5 gene

Methodology Applied

Scientific EffectHybridization:

Implementation Method 2

an antibody that recognizes the 187A5 protein

Methodology Applied

Scientific EffectAntigen-antibody binding:

Data Source

PatentEP2006382B1Dopamine-producing neuron progenitor cell marker 187a5

Publication Date: 2016.08.03 EISAI R&D MANAGEMENT CO LTD

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AI summary

An object of the present invention is to provide a probe, a primer, a primer set and an antibody for use in the detection or selection of a dopaminergic neuron progenitor cell. The present invention provides a probe, a primer and a primer set for use in the detection or selection of a mesencephalon dopaminergic neuron progenitor cell, and preferably a dopaminergic neuron proliferative progenitor cell, which can hybridize with a nucleotide sequence of a 187A5 gene, or a complementary sequence thereto, and an antibody for use in the detection or selection of a mesencephalon dopaminergic neuron progenitor cell, and preferably a dopaminergic neuron progenitor cell, which is capable of binding to a 187A5 protein.