1PLRF93 Backcross Conversion for Stable Maize Breeding
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Solution Overview
Problem
The development of new maize varieties is a time-consuming process that requires years to combine desirable traits such as disease resistance, drought tolerance, and high yield, posing challenges in efficiently producing stable and agronomically sound maize varieties.
Innovation Solution
The introduction of the novel maize variety 1PLRF93, which can be bred through backcross conversion, genetic manipulation, and transformation, incorporating traits like male sterility, disease resistance, and improved agronomic characteristics, along with processes for crossing and hybrid seed production, to create genetically stable and high-yielding maize plants.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional maize variety development processes are used to combine desirable traits, then the resulting varieties have stable and agronomically sound characteristics, but the development time is excessively long (6-12 years)
Solution Approach 1:
The patent applies preliminary action by pre-establishing standardized inbred lines with known genetic characteristics and creating a library of pre-characterized parental varieties. This allows breeders to start hybrid development with already-validated genetic material, eliminating the need to develop new inbreds from scratch for each hybrid program, thus significantly reducing the 6-12 year development timeline while maintaining variety stability through proven genetic backgrounds.
Solution Approach 2:
The patent segments the maize breeding process into distinct modular components: inbred line development, hybrid combination testing, and variety registration. By segmenting the process and developing inbred lines independently with standardized protocols, the system allows parallel development of multiple parental lines that can be systematically combined, reducing overall development time while maintaining the reliability of each segment through standardized evaluation criteria.
2Productivity
If multiple desirable traits are combined in a single variety through traditional breeding, then the variety achieves high yield and agronomic soundness, but the breeding process becomes increasingly complex and time-consuming
Solution Approach 1:
The patent applies universality by developing inbred lines that serve multiple functions: they can be used as parental lines for hybrid production, as test entries for variety trials, and as genetic resources for future breeding programs. The standardized inbred lines are characterized for multiple traits simultaneously (yield, disease resistance, maturity, stress tolerance), allowing a single breeding effort to produce materials that address multiple productivity requirements without proportionally increasing process complexity.
Solution Approach 2:
The patent systematically changes and optimizes key breeding parameters including inbred line homozygosity levels, hybrid combination ratios, and trial replication numbers. By establishing standardized parameter ranges for inbred line development (e.g., minimum 6 generations of selfing for homozygosity) and hybrid testing, the system achieves consistent high-yielding varieties while controlling process complexity through documented parameter specifications rather than ad hoc breeding decisions.
3Reliability
If traditional maize breeding methods are used, then desirable traits are successfully combined, but the process requires extensive manual labor and field operations over multiple years
Solution Approach 1:
The patent replaces manual mechanical breeding operations with molecular marker-assisted selection and DNA-based variety identification systems. Instead of relying solely on phenotypic observation and manual cross-pollination over multiple generations, the system uses molecular markers to track desirable traits through generations and verify hybrid authenticity, significantly reducing the time required to develop and validate new varieties while maintaining reliable trait combination through genetic marker verification.
Solution Approach 2:
The patent implements feedback mechanisms through systematic variety performance testing and molecular marker verification at each breeding stage. Field trial data on yield, disease resistance, and other traits are fed back into the breeding program to guide selection decisions, while molecular markers provide immediate feedback on genetic composition. This continuous feedback loop accelerates the breeding cycle by enabling informed selection decisions without waiting for complete phenotypic expression, reducing the 6-12 year timeline while ensuring reliable trait combination.
Data Source
AI summary
A novel maize variety designated 1PLRF93 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety 1PLRF93 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 1PLRF93 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety 1PLRF93 or a locus conversion of 1PLRF93 with another maize variety.