Maize Inbred 1PPFS42 Using Doubled Haploids for Stable Hybrids
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Solution Overview
Problem
The development of new maize varieties is a time-consuming process that requires years to combine desirable traits such as disease resistance, drought tolerance, and high yield, posing challenges in efficiently producing stable and agronomically sound maize varieties.
Innovation Solution
The introduction of the novel maize variety 1PPFS42, which incorporates genetic modifications and cytoplasmic traits like male sterility, along with processes for crossing and hybrid production, enables the development of maize plants with enhanced traits like herbicide tolerance, disease resistance, and improved agronomic characteristics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If traditional breeding methods are used to develop new maize varieties, then desirable traits such as disease resistance, drought tolerance, and high yield can be combined, but the process is time-consuming and takes six to twelve years from the first cross to finished seed delivery
Solution Approach 1:
The patent applies preliminary action by using doubled haploid technology to create inbred lines in a single generation rather than requiring multiple generations of selfing. This preliminary creation of homozygous lines accelerates the breeding process while maintaining the stability and reliability of the final variety, reducing development time from six to twelve years to a significantly shorter duration.
Solution Approach 2:
The patent changes the temporal parameter of the breeding process by utilizing molecular markers and genomic selection to evaluate and select lines at earlier stages. This parameter change allows for accelerated selection and evaluation, reducing the time required to develop stable, high-yielding varieties while maintaining agricultural performance.
2Adaptability or versatility
If multiple desirable traits are combined in a single variety through traditional breeding, then agronomic soundness and yield stability are improved, but the complexity of the breeding process increases
Solution Approach 1:
The patent applies segmentation by dividing the complex breeding process into distinct modular steps: creation of inbred lines via doubled haploids, evaluation using molecular markers, selection of superior lines, and final variety development. This segmentation reduces the overall complexity by making each step manageable and independent, while still achieving the integration of multiple desirable traits for agronomic soundness.
Solution Approach 2:
The patent introduces molecular markers as intermediaries to facilitate the breeding process. These markers serve as mediators between the desired traits and the selection process, enabling accurate identification and selection of lines with multiple desirable traits without requiring complex phenotypic evaluations, thus reducing breeding process complexity while maintaining agronomic versatility.
3Stability of the object's composition
If inbred lines are created through multiple generations of selfing, then homozygosity and genetic stability are achieved, but the time required for line development increases significantly
Solution Approach 1:
The patent replaces the mechanical biological process of repeated selfing and natural homozygosity with a laboratory-based doubled haploid system. This substitution uses in vitro fertilization and chromosome doubling to achieve homozygosity in a single generation, eliminating the need for multiple generations of selfing and dramatically reducing line development time while maintaining genetic stability.
Data Source
AI summary
A novel maize variety designated 1PPFS42 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety 1PPFS42 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 1PPFS42 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety 1PPFS42 or a locus conversion of 1PPFS42 with another maize variety.