2D Spectroscopy System Using Time-Delayed Pulse Arrays
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Solution Overview
Problem
Current 2D spectroscopy systems face challenges in identifying biomolecular characteristics due to broad THz absorption spectra, requiring improved methods to reduce data acquisition time and enhance spectral resolution.
Innovation Solution
A 2D spectroscopy system and method that utilize a pulsed light source, beam splitter, light transmission delayer, response pulse wave generator, optical readout pulse array generator, reader, image detector, and signal processor to generate and process response pulse waves with relative time delays, reducing scanning time and enhancing spectral analysis through Fourier transforms of absorption and emission spectra.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If THz absorption spectroscopy is used to measure low frequency vibrational modes, then biomolecular characteristics can be identified, but the broad absorption spectra make it difficult to resolve specific characteristics
Solution Approach 1:
The patent transforms the conventional one-dimensional frequency spectrum into a two-dimensional spectrum by introducing a second frequency dimension through dual pulse excitation. This dimensional transformation allows resolution of broad spectral features by distributing overlapping signals across two dimensions, enabling separation of closely spaced vibrational modes that cannot be distinguished in conventional 1D spectra.
Solution Approach 2:
The patent segments the excitation process into multiple discrete light pulses with controlled time delays, rather than using continuous or single-pulse excitation. This segmentation allows the spectral information to be distributed across multiple time points, enabling Fourier transformation to resolve overlapping frequency components that appear broad in conventional measurements.
2Measurement precision
If conventional 2D spectroscopy methods are used, then spectral resolution is improved, but data acquisition time is excessively long due to scanning of optical readout pulses
Solution Approach 1:
The patent performs preliminary action by pre-forming a complete optical readout pulse array with all necessary time delays before the measurement process. This allows simultaneous readout of multiple time points in a single shot, eliminating the need for sequential scanning during data acquisition and dramatically reducing measurement time while maintaining 2D spectral resolution.
Solution Approach 2:
The patent implements continuous useful action by using a pulsed laser source that continuously generates light pulses to form the complete readout pulse array. This continuous pulsed operation allows all necessary readout pulses to be available simultaneously for a single measurement, eliminating idle scanning time and maintaining uninterrupted data collection.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach significantly reduces data acquisition time, improves signal-to-noise ratio, and enables detailed analysis of biomolecular characteristics, including the identification and quantification of biomolecules, facilitating disease screening and diagnosis.
Implementation Method 1
a beam splitter configured to split light pulses provided from the pulsed light source into first light pulse and second light pulse
Implementation Method 2
a light transmission delayer configured to form a plurality of first light pulses from the first light pulse and to cause a first relative time delay with respect to the plurality of first light pulses
Implementation Method 3
a response pulse wave generator configured to generate a plurality of response pulse waves having a wavelength range to which a sample to be analyzed responds by using the plurality of first light pulses sequentially inputted from the light transmission delayer
Implementation Method 4
The reader may include an electro-optic (EO) crystal configured to read out a signal by performing EO sampling which includes overlapping the signal generated from the sample with the optical readout pulse array
Implementation Method 5
acquire spectroscopic analysis information which relates to the sample by processing the signal image detected by the image detector, including the identification and quantification of the biomolecule, through Fourier transforms of absorption and emission spectra
Data Source
AI summary
A two-dimensional (2D) spectroscopy system and a 2D spectroscopic analysis method are disclosed. The 2D spectroscopy system includes: a light transmission delayer configured for forming a plurality of first light pulses from first light pulse and causing a relative time delay therebetween; a response pulse wave generator configured for generating a plurality of response pulse waves responds and having a relative time delay, and for irradiating the plurality of response pulse waves on the sample; an optical readout pulse array generator configured for forming an optical readout pulse array by splitting the second light pulse into a plurality of regions having different time delays and spatially discriminated from one another; and a reader configured for reading out by overlapping the optical readout pulse array with a signal generated from the sample.