2′-FL Crystallization Using Acetic Acid Seeding for Better Filtration
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Solution Overview
Problem
Existing crystallization methods for 2′-O-fucosyllactose (2′-FL) consume a high amount of antisolvent and produce crystals with suboptimal properties such as unstable morphology, poor filterability, high moisture content, and longer drying times, while maintaining good yield and purity.
Innovation Solution
A method involving the use of a suspension of crystalline 2′-FL polymorph II in acetic acid added to an aqueous solution containing 2′-FL, followed by the addition of acetic acid to form a slurry and subsequent filtration, reducing the amount of acetic acid used while maintaining high yield and purity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional crystallization methods using high amounts of acetic acid are used, then purity is maintained, but the amount of antisolvent consumed increases and crystal properties deteriorate
Solution Approach 1:
The invention changes the physical-chemical parameters of the crystallization process by using a controlled amount of acetic acid (1-10% w/w) rather than large excess amounts. This parameter optimization allows crystallization to proceed while maintaining stable crystal morphology and improving filterability, resolving the contradiction between antisolvent consumption and crystal quality
Solution Approach 2:
The invention applies preliminary action by adding a suspension of crystalline 2'-FL polymorph II in acetic acid to the aqueous solution before completing the crystallization process. This seeding approach initiates controlled crystal formation early in the process, ensuring stable morphology and reducing the total acetic acid needed while maintaining purity
2Quantity of substance
If conventional crystallization methods are used, then purity is maintained, but filterability deteriorates
Solution Approach 1:
By optimizing the acetic acid concentration to 1-10% w/w and controlling the crystallization conditions, the invention produces crystals with improved physical properties including better filterability. The controlled parameter changes prevent excessive crystal aggregation and maintain pore structure in the crystal mass, resolving the contradiction between antisolvent usage and operational ease
3Quantity of substance
If conventional crystallization methods are used, then purity is maintained, but drying time increases
Solution Approach 1:
The invention reduces acetic acid content in the crystallization system to 1-10% w/w, which directly reduces the volatile content in the crystal mass. This parameter change accelerates the drying process by reducing the amount of solvent that needs to be evaporated, thereby resolving the contradiction between antisolvent consumption and processing time
Solution Approach 2:
By adding the crystalline suspension in advance and controlling the crystallization process early, the invention produces crystals with lower inherent moisture and volatile content. This preliminary controlled crystallization reduces the subsequent drying burden, resolving the time loss contradiction
4Quantity of substance
If conventional crystallization methods are used, then yield is maintained, but moisture content increases
Solution Approach 1:
The invention optimizes acetic acid concentration to 1-10% w/w and controls crystallization parameters to produce crystals with lower moisture and volatile content. This parameter optimization improves the quality of the crystalline product, reducing post-processing requirements and enhancing overall productivity while maintaining high yield
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method produces 2′-FL polymorph II with improved crystal morphology, better filterability, lower moisture and volatile content, and faster drying, using significantly less acetic acid compared to prior art methods, with a yield of at least 70% and purity of at least 97%, suitable for nutritional and pharmaceutical compositions.
Implementation Method 1
adding a suspension of crystalline 2'-FL, advantageously polymorph II of 2'-FL, in acetic acid to the aqueous solution or syrup comprising 2'-FL, thereby generating a slurry, adding acetic acid to the slurry to obtain a crystalline mass
Data Source
AI summary
The invention relates to a method for selective crystallization of 2′-FL from an aqueous solution comprising 2′-FL and one or more other fucosylated carbohydrates by adding acetic acid to the solution.