3D Cell Structure Culture Chamber for Suturable Tissue Strength
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Solution Overview
Problem
Conventional three-dimensional cell structures and artificial skins lack the strength necessary for suturing, limiting their applications in transplantation and other uses.
Innovation Solution
A method involving the three-dimensional culture of original cells, including fibroblasts, in a culture chamber with a specific configuration that allows medium circulation, leading to the formation of a three-dimensional cell structure with increased breaking force by growing it beyond the chamber's boundaries.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Strength
If conventional methods are used to produce three-dimensional cell structures, then the production process is simple, but the resulting structures do not have sufficient strength for suturing
Solution Approach 1:
The culture chamber is divided into multiple cavities, each capable of independently producing three-dimensional cell structures. This segmentation allows for standardized production units that can be scaled while maintaining structural integrity and strength requirements.
Solution Approach 2:
The invention transitions from two-dimensional cell culture to three-dimensional cell structure formation by utilizing cavity-based chambers that enable cells to grow in all spatial dimensions, producing integrated structures with sufficient breaking force for suturing applications.
2Strength
If three-dimensional cell structures are cultured to achieve sufficient strength, then the breaking force increases to 0.08 N or more, but the culture process becomes more complex
Solution Approach 1:
The culture chamber is pre-configured with specific cavity structures before cell introduction. This preliminary preparation ensures that cells will naturally form integrated three-dimensional structures with sufficient strength during the culture process, eliminating the need for post-culture structural reinforcement or complex manipulation steps.
Solution Approach 2:
The culture system enables cells to automatically form integrated three-dimensional structures with adequate breaking force through the natural culture process within the cavity chamber, without requiring external intervention or additional processing steps to achieve the required strength.
3Stability of the object's composition
If original cells are cultured to form integrated three-dimensional structures, then the structural integrity improves, but the culture time and space requirements increase
Solution Approach 1:
The culture chamber is divided into multiple cavities, each producing individual three-dimensional cell structures. This segmentation allows for efficient use of space while maintaining the structural integrity of each produced structure, as each cavity acts as an independent production unit.
Solution Approach 2:
Each cavity within the culture chamber is designed with specific local characteristics optimized for three-dimensional cell structure formation. This localized optimization ensures that each region of the chamber efficiently produces structurally integrated cell structures with appropriate breaking force.
Data Source
Figure 1
Figure 2(a)~2(c)
Figure 3A~3B
AI summary
The purpose of the present invention is to provide a three-dimensional cell structure having strength that enables suturing. This method for producing a three-dimensional cell structure includes: (1) a step for preparing original cells having proliferation capability, and a culture chamber which includes a cavity having an internal space surrounded by a lower surface, an upper surface, and side surfaces, and which is formed such that the culture liquid can circulate in the internal space; (2) a step for supplying the original cells to the culture chamber; (3) a step for holding the whole culture chamber in a culture liquid; and (4) a step for culturing the original cells, integrating the original cells, the cultured cells derived therefrom, and an extracellular matrix produced during the culturing, and forming a three-dimensional cell structure that has grown to the outside of the cavity.