3D Cell Culture Container With Guided Injection and Pillar Height Control
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Solution Overview
Problem
Existing cell culture devices face challenges in accurately injecting a predetermined amount of cells, ensuring uniform distribution, and maintaining consistent height for effective three-dimensional cell culture observation, particularly in devices with complex injection methods and varying culture environments.
Innovation Solution
A cell culture container featuring a lower plate with pillars, an upper plate, and injection guides that facilitate controlled cell injection, uniform distribution, and stable material exchange, using materials like glass, silicone rubbers, and engineering plastics, with pillar gaps and diameters optimized for secure seating and material transfer.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If a conventional cell culture container is used, then the device structure is simple, but the injection precision of cell sample amount and height cannot be controlled
Solution Approach 1:
The container is divided into functional components: injection guide parts with hole structures for precise cell sample injection, pillar structures for height control, and barrier parts for containment. Each component addresses a specific precision requirement, enabling controlled injection of predetermined amount and height while maintaining reasonable structural complexity
Solution Approach 2:
The injection guide parts act as intermediaries between the injection device and the cell sample, providing a structured interface that guides and controls the injection process. The hole structures in the injection guide parts serve as mediators to achieve precise delivery of cell samples at predetermined locations and heights
2Manufacturing precision
If cells are injected without guidance structures, then the device complexity is low, but the uniformity of cell distribution and height consistency deteriorate
Solution Approach 1:
The injection guide parts and pillar structures are pre-formed with specific geometries before the cell injection process. The hole structures in injection guide parts and the pillar arrangements are designed in advance to automatically guide and control the injection process, ensuring uniform cell distribution and consistent height without requiring complex real-time control mechanisms
Solution Approach 2:
The pillar structures and barrier parts automatically perform the function of containing and positioning the cell sample through their physical geometry. The system uses its own structural features to achieve height consistency and uniform distribution, eliminating the need for external control mechanisms
3Reliability
If the container lacks specific injection guide parts, then the ease of manufacture is high, but the reliability of experimental results deteriorates
Solution Approach 1:
The container is segmented into modular components (injection guide parts, barrier parts, pillar structures) that can be manufactured separately and assembled. This segmentation enables complex functional features to be produced using standard manufacturing processes, maintaining reliability while managing manufacturing complexity
Solution Approach 2:
The injection guide parts incorporate hole structures with specific dimensional parameters that control the injection process. By optimizing parameters such as hole diameter, depth, and positioning, the system achieves reliable cell sample delivery while maintaining compatibility with conventional manufacturing techniques
Data Source
AI summary
The present invention relates to a three-dimensional cell culture container. A cell culture container according to the present invention may include: a barrier part composed of two or more pillars (110) provided on the top side of the lower plate; an upper plate (130) which finishes an opened end portion of the barrier part; and one or more cell sample injection guide parts (220) provided in one side of the upper plate. The present invention using such configurations can stably inject a sample including cells into an accurate position in a state that the sample leans on the guide parts, and the inlet guide may perform a barrier function such that a flow of the sample is directed toward the culture container, and the upper plate and the plurality of pillars facilitate an operation of an experimenter by performing a helping function such that an appropriate amount of sample is injected easily and uniformly, while the pillar parts spaced apart from one another at predetermined intervals enable a stable cell culturing process to be performed by performing a barrier function of preventing a gel-type sample from being discharged to the outside and performing a function of opening a space for exchanging materials with a medium in an external cell.