3D Target Cell Extraction With Preserved Spatial Context
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Solution Overview
Problem
Current methods for analyzing solid tumors in 2D slices fail to capture the three-dimensional structure and spatial relationships of cells within tissues, leading to loss of morphological information and hindering personalized immune cell therapy approaches.
Innovation Solution
A method involving optical imaging and spatial parameter registration to identify and extract target cells from 3D specimens, using techniques like light sheet microscopy and confocal microscopy, followed by encapsulation or tagging to preserve spatial information, enabling further analysis without disassembly.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If tissue is dissociated into single cells for analysis, then individual cell analysis becomes possible, but spatial and morphological information is completely lost
Solution Approach 1:
The patent applies preliminary action by performing optical identification and spatial parameter registration of target cells within the intact 3D tissue structure before any dissociation occurs. This ensures that spatial context information is captured and preserved in a database before the tissue is broken down into single cells for subsequent analysis.
Solution Approach 2:
The patent creates an optical copy or digital representation of the 3D tissue structure with all spatial relationships intact. By registering spatial parameters and creating a database of target cell locations within this optical copy, the system preserves spatial information in digital form while allowing physical dissociation for analysis.
2Ease of operation
If 2D slices are used for tissue analysis, then analysis simplicity is maintained, but three-dimensional structure and spatial relationships are not captured
Solution Approach 1:
The patent transitions from 2D slice analysis to 3D optical imaging, capturing tissue architecture and spatial relationships in three dimensions. By using light sheet microscopy or confocal microscopy to image the entire 3D specimen and registering spatial parameters (x, y, z coordinates), the system maintains operational simplicity while preserving all three-dimensional structural information.
3Loss of information
If complete 3D reconstruction with cellular resolution is performed, then full spatial information is obtained, but the process becomes extremely time-consuming and cumbersome
Solution Approach 1:
The patent extracts only the essential spatial information needed for the analysis by registering spatial parameters of specific target cells rather than performing complete 3D reconstruction of all tissue structures. This selective extraction of spatial data from the 3D optical images significantly reduces processing time while maintaining the spatial context necessary for accurate analysis.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables precise extraction and analysis of target cells with preserved spatial context, facilitating personalized therapies by maintaining morphological and intercellular environment information.
Implementation Method 1
imaging the three dimensional specimen
Implementation Method 2
using techniques like light sheet microscopy and confocal microscopy
Implementation Method 3
registering the spatial parameters (x,y,z coordinates) of the target cells
Data Source
AI summary
The invention is directed to a process for extracting target cells from a three dimensional biological specimen by the steps imaging the three dimensional specimen; identifying target cells; registering the spatial parameters (x,y,z coordinates) of the target cells; and extraction of target cells according to their spatial parameters