3D Microneedle Microelectrode Arrays for Multiplexed Cell Sensing
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Solution Overview
Problem
Existing 3D microelectrode arrays (MEAs) lack the capability to simultaneously sense multiple physiological parameters such as temperature and analytes, and provide comprehensive data sets for complex cellular models, necessitating improved multiplexed systems for in vitro electrophysiological studies.
Innovation Solution
A 3D microelectrode array device with integrated temperature sensing electrodes and analyte sensing interdigitated electrodes, along with microfluidic ports, microneedles, and metallic traces, fabricated using polymers like polycarbonate and gold layers, enabling simultaneous sensing and stimulation of multiple parameters.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If traditional 3D microelectrode arrays are used, then electrophysiological sensing is achieved, but multiple sensing capabilities (temperature, analytes) are not provided
Solution Approach 1:
The patent implements multi-functionality by integrating temperature sensing electrodes, analyte sensing interdigitated electrodes, and electrophysiological sensing microelectrodes within a single 3D MEA device platform. This allows one device to perform multiple sensing functions simultaneously, resolving the contradiction between versatility improvement and device complexity increase.
Solution Approach 2:
The patent combines previously separate sensing systems (electrophysiological MEAs, temperature sensors, analyte sensors) into a unified integrated device. By merging these functions into a single platform with common substrate and coordinated electrode arrangements, the patent achieves multi-modal sensing without proportionally increasing overall device complexity.
2Loss of information
If single-parameter sensing is used, then device simplicity is maintained, but comprehensive data sets for complex cellular models cannot be procured
Solution Approach 1:
The integrated 3D MEA device provides multi-functional sensing capabilities including electrophysiological parameters, temperature, and analyte concentrations, enabling comprehensive data collection for complex cellular models without requiring multiple separate devices.
Solution Approach 2:
The patent segments the sensing functions into distinct electrode types (microelectrodes for electrophysiology, temperature sensing electrodes, interdigitated electrodes for analytes) that operate simultaneously within a unified device, allowing comprehensive data collection while maintaining organized device architecture.
3Productivity
If multiple sensing modalities are integrated, then multiplexed data collection is achieved, but fabrication complexity increases
Solution Approach 1:
The patent combines multiple sensing modalities into a single integrated fabrication process, where temperature sensing electrodes, analyte sensing interdigitated electrodes, and electrophysiological microelectrodes are manufactured together on the same substrate using coordinated fabrication steps.
Solution Approach 2:
The unified device platform performs multiple sensing functions simultaneously, enabling multiplexed data collection that increases productivity without requiring separate fabrication processes for each sensing modality.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device achieves multi-modal sensing capabilities, including electrophysiology, temperature sensing, analyte detection, and microfluidic perfusion, providing comprehensive data collection for complex cellular models, enhancing biological research.
Implementation Method 1
A microheater may be positioned on the bottom face of the substrate
Implementation Method 2
A temperature sensing electrode may be formed on the top face adjacent the microheater positioned on the bottom face
Implementation Method 3
An analyte sensing electrode may be formed on the top face adjacent the 3D microneedle array
Data Source
AI summary
A three-dimensional (3D) microelectrode array device for in vitro electrophysiological applications includes a substrate and micro vias extending from the bottom face to the top face of the substrate. A microneedle at each micro via extends from the bottom face upward beyond the top face and forms a hypodermic microneedle array on the top face. Metallic traces on the bottom face interconnect the hypodermic microneedles to form the 3D microelectrode array. A microheater is positioned on the bottom face of the substrate. Microfluidic ports may be formed at the substrate. Interdigitated electrodes may be formed at the substrate.