3D Microparticle Data Analysis Apparatus
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Solution Overview
Problem
Conventional data analysis apparatuses for flow cytometers require users to refer to multiple histograms or cytograms to specify microparticle populations, making the gating process cumbersome, especially with increased parameters and the need to visualize three-dimensional distribution charts.
Innovation Solution
A 3D data analysis apparatus that stores measurement data, allows selection of three independent variables, calculates positions and graphics in a coordinate space, and displays a 3D stereoscopic image representing the characteristic distribution of microparticles, enabling intuitive analysis without relying on numerous histograms or 3D charts.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If multiple histograms or cytograms are used to specify microparticle populations, then measurement precision is improved, but device complexity and ease of operation deteriorate
Solution Approach 1:
The patent combines multiple measurement parameters (forward scattered light, side scatter, fluorescent light, impedance) into a single three-dimensional distribution chart where the X, Y, and Z axes represent different parameters. This merging of multiple graphs into one integrated 3D visualization allows users to specify microparticle populations with high precision without needing to refer to multiple separate histograms or cytograms, thereby reducing device complexity while maintaining measurement accuracy.
Solution Approach 2:
The patent transitions from two-dimensional histograms and cytograms to a three-dimensional distribution chart by adding a third dimension to visualize multiple measurement parameters simultaneously. This dimensional expansion enables the display of four parameters (FS, SS, FL, impedance) in a single 3D space, improving ease of operation and reducing the number of graphs needed while preserving the ability to precisely specify microparticle populations through spatial positioning in the 3D chart.
2Measurement precision
If multiple histograms or cytograms are referred to for gating, then measurement precision is improved, but ease of operation deteriorates
Solution Approach 1:
The patent merges multiple measurement parameters into a single three-dimensional distribution chart, allowing users to perform gating by specifying a single region in 3D space rather than coordinating across multiple separate graphs. This integration maintains the precision needed for accurate cell population selection while dramatically improving ease of operation by eliminating the need to switch between or correlate multiple histograms and cytograms during the gating process.
Solution Approach 2:
By introducing a third dimension in the distribution chart, the patent enables users to visualize and gate on four measurement parameters (forward scattered light, side scatter, fluorescent light, and impedance) simultaneously in one view. This 3D visualization simplifies the gating operation to a single intuitive action while preserving measurement precision, as users can directly position gates in the three-dimensional parameter space without the complexity of coordinating multiple 2D graphs.
3Loss of information
If three-dimensional distribution charts are visualized conventionally, then information completeness is improved, but ease of operation deteriorates
Solution Approach 1:
The patent utilizes a three-dimensional coordinate system where the X, Y, and Z axes represent different measurement parameters (forward scattered light, side scatter, and fluorescent light or impedance). This 3D visualization displays multifactorial data complete in a single chart, and by leveraging human spatial perception, it makes the data more intuitive to interpret than traditional 2D representations, thereby improving ease of operation while maintaining information completeness.
Solution Approach 2:
The patent employs color coding to represent the fourth measurement parameter (impedance or fluorescent light intensity) through variations in point color or brightness within the three-dimensional distribution chart. This visual encoding allows users to perceive multiple parameters simultaneously without increasing chart complexity, making the 3D distribution chart easier to interpret while preserving complete multifactorial information.
Data Source
AI summary
An information processing apparatus for 3D microparticle data analysis is provided. The information processing apparatus includes a data storage unit configured to store measurement data of microparticles; a data processing unit configured to create a 3D image in a coordinate space with three types of variables from the measurement data, the 3D image represents a characteristic distribution of the microparticles; and a display unit configured to display the 3D image, wherein in a case that a gating region is set in the 3D image, the 3D image is rotated or scaled up or down along with the gating region on the display unit. An information processing method and program for 3D microparticle data analysis are also provided.