5-FU Pathway Probe Composition for Gene Copy Number Prediction
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Solution Overview
Problem
Current cancer treatments, particularly those involving 5-fluorouracil (5-FU), face challenges in predicting patient response and identifying resistance, leading to unnecessary side effects and resource inefficiencies due to the lack of effective predictive markers.
Innovation Solution
Development of a composition and method using probes to detect specific markers within the 5-FU pathway, such as Thymidylate synthase (TYMS), Dihydrofolate reductase (DHFR), Thymidine phosphorylase (TP), Dihydropyrimidine dehydrogenase (DPD), Methylenetetrahydrofolate reductase (MTHFR), and Thymidine kinase (TK), to determine gene copy number changes and predict treatment outcomes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If cytotoxic chemotherapy is administered to cancer patients, then treatment efficacy is improved, but unnecessary side effects occur due to inability to predict patient response
Solution Approach 1:
The invention performs preliminary detection of gene copy number changes in 5-FU pathway markers (TYMS, DHFR, TP, DPD, MTHFR, TK) before administering chemotherapy. This advance assessment allows clinicians to predict patient response to 5-FU treatment and avoid administering the drug to patients who are likely to experience resistance or excessive toxicity, thereby preventing unnecessary side effects while maintaining treatment efficacy for responsive patients.
2Measurement precision
If multiple markers are detected to improve prediction accuracy, then measurement precision is improved, but device complexity increases
Solution Approach 1:
The invention segments the complex detection task into individual probe-target pairs for each marker (TYMS, DHFR, TP, DPD, MTHFR, TK). Each probe is designed to specifically bind to its corresponding marker's DNA sequence, allowing independent detection and quantification of gene copy number changes. This segmentation approach enables accurate multi-marker analysis while maintaining manageable system complexity through modular probe design and separate hybridization reactions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for precise prediction of 5-FU treatment response and resistance, reducing side effects and optimizing treatment by identifying gene aberrations in cancer cells before therapy initiation.
Implementation Method 1
a composition comprising at least two probes binding to at least two markers from the 5-FU pathway
Data Source
AI summary
This invention is directed to compositions and their uses for detection of markers. Such markers may be useful in the understanding of the underlying molecular event leading to a condition or a disease in a subject. These markers may also be useful for characterization of neoplastic cells and cancer cells and their response to certain therapeutical regimes. Therefore the invention as disclosed may contribute to the improvement of the stratification of patients for the best possible treatment.