AAD-1 Corn Event DAS-40278-9 Transgene Expression
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Solution Overview
Problem
The challenge in plant breeding is achieving consistent and desired levels of transgene expression in crops, as the insertion site of foreign genes in the chromosome influences expression, leading to variability in expression levels and patterns across different plant tissues, necessitating the creation and screening of numerous events to identify suitable ones for commercial use.
Innovation Solution
The development of the AAD-1 corn event DAS-40278-9, which includes a specific insertion of the aad-1 polynucleotide sequence into a targeted site in the corn genome, allowing for herbicide tolerance and enabling the stacking of additional traits, along with methods for detecting the event using unique DNA sequences and assays.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If foreign genes are inserted into the chromosome, then transgene expression is achieved, but expression levels vary widely depending on insertion site
Solution Approach 1:
The patent employs parameter changes by modifying the insertion site parameters (chromosomal location, genomic context) to achieve desired expression levels. Multiple events with different insertion parameters are created and screened to find optimal expression consistency and levels for commercial use.
2Reliability
If large numbers of events are created and screened, then suitable events with desired expression levels are identified, but time and resource consumption increase
Solution Approach 1:
The patent applies preliminary action by pre-screening and characterizing multiple transformation events during the development phase to identify those with stable and desirable expression patterns before commercial deployment. This preliminary characterization reduces the need for extensive field testing and accelerates regulatory approval.
3Productivity
If transgene expression levels are optimized, then herbicide tolerance is achieved, but variability in spatial and temporal expression patterns persists
Solution Approach 1:
The patent applies local quality by accepting and working with different spatial and temporal expression patterns in different tissues and developmental stages. Rather than requiring uniform expression everywhere, the invention identifies events that achieve sufficient tolerance levels in critical tissues while maintaining overall crop performance.
Data Source
AI summary
This invention relates in part to plant breeding and herbicide tolerant plants. This invention includes a novel aad-1 transformation event in corn plants comprising a polynucleotide sequence, as described herein, inserted into a specific site within the genome of a corn cell. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. Additionally, the subject invention provides assays for detecting the presence of the subject event in a sample (of corn grain, for example). The assays can be based on the DNA sequence of the recombinant construct, inserted into the corn genome, and on the genomic sequences flanking the insertion site. Kits and conditions useful in conducting the assays are also provided. The subject invention also includes related methods of controlling weeds.