ABC Transporter ATP-Binding Protein Variant for L-Lysine Production
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Solution Overview
Problem
Current methods for producing L-lysine using Corynebacterium glutamicum strains are not sufficiently efficient, as they do not effectively enhance the microorganism's ability to produce this amino acid.
Innovation Solution
A variant of the ABC transporter ATP-binding protein is introduced, where valine at position 199 is substituted with methionine, and a corresponding polynucleotide is used to create a Corynebacterium glutamicum strain that exhibits increased L-lysine production capabilities.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional Corynebacterium glutamicum strains are used for L-lysine production, then the production process is simple, but the L-lysine production yield is insufficient
Solution Approach 1:
The patent applies parameter changes by substituting a specific amino acid (valine at position 199 with methionine) in the ABC transporter ATP-binding protein sequence, which alters the protein's functional properties and thereby increases L-lysine production yield without requiring complex structural modifications to the overall production system
Solution Approach 2:
The invention implements local quality by making a targeted local modification (single amino acid substitution) in a specific protein region rather than globally modifying the entire organism or protein structure, thereby achieving enhanced L-lysine production with minimal changes to the strain's overall characteristics
2Productivity
If gene expression is increased to enhance L-lysine production, then productivity improves, but metabolic balance and cell growth may be disrupted
Solution Approach 1:
Instead of increasing gene expression levels, the patent changes the functional parameter of the transport protein through amino acid substitution, which improves L-lysine production capability while maintaining normal metabolic balance and cellular physiological states
Data Source
Figure 1
AI summary
The present disclosure relates to a novel ABC transporter ATP-binding protein variant, a Corynebacterium glutamicum strain comprising the variant, and a method for producing L-lysine using the strain.