ABCB5 Stem Cell Isolation for Ocular Regeneration

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Solution Overview

Problem

Current treatments for ocular conditions such as corneal and retinal diseases lack effective regenerative solutions, particularly for conditions like limbal stem cell deficiency and macular degeneration, where the ability to regenerate ocular cells is limited.

Innovation Solution

The use of ABCB5(+) stem cells, specifically ABCB5-positive limbal stem cells and retinal pigment epithelium cells, which are isolated and administered to promote ocular cell regeneration by seeding them onto substrates like fibrin gel or artificial corneas for transplantation.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional treatments are used for ocular conditions, then current standard therapies are applied, but effective regenerative solutions are lacking

Engineering Contradiction:

Improveeffectiveness of treatmentVSAvoidregenerative capability

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent extracts and isolates specific stem cell populations (ABCB5-positive cells) from mixed ocular cell populations using flow cytometry and sorting techniques. This extraction of the regenerative component enables targeted transplantation to treat ocular conditions, directly addressing the lack of effective regenerative solutions in conventional treatments

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent utilizes the ABCB5 surface marker expression parameter to identify, isolate, and sort stem cells from mixed populations. By changing the selection parameter from conventional markers to ABCB5, the patent achieves superior isolation of regenerative cells, thereby improving both treatment reliability and regenerative capability

Inventive Principle:

Principle #35Parameter changes

2Reliability

If stem cells are administered to promote regeneration, then ocular cell regeneration is enhanced, but isolation from mixed populations becomes necessary

Engineering Contradiction:

Improveregeneration effectivenessVSAvoidisolation process complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent employs fluorescently labeled antibodies that bind to ABCB5 markers on stem cells, causing them to emit detectable fluorescent signals. This optical property change enables automated flow cytometry-based sorting and isolation of stem cells from mixed populations, achieving reliable regeneration enhancement while managing isolation complexity through automated optical detection

Inventive Principle:

Principle #32Color changes

Solution Approach 2:

The patent replaces manual or mechanical cell separation methods with automated flow cytometry and magnetic-activated cell sorting (MACS) systems. This substitution uses fluid dynamics and magnetic fields to isolate ABCB5-positive cells, reducing manual complexity while maintaining high regeneration effectiveness

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS20220118024A1ABCB5(+) stem cells for treating ocular disease

Publication Date: 2022.04.21 CHILDRENS MEDICAL CENT CORP

AI summary

Various aspects and embodiments of the present invention are directed to methods of treating a subject having an ocular condition, methods of isolating ocular stem cells, methods of selecting and/or producing ocular grafts for transplantation, and methods of promoting ocular cell regeneration as well as to grafts and preparations containing isolated ocular stem cells characterized by the expression of ABCB5 on their cell surface.