Absorbent Particles Reduce Light Interference in Spot Analysis
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Solution Overview
Problem
Current analysis methods face challenges in accurately detecting analytes in a liquid phase due to light interference, leading to false positive and false negative results, particularly in multiplex analysis methods using chemiluminescence, where background noise from the liquid phase interferes with signal detection.
Innovation Solution
The use of absorbent particles, such as carbon particles, is introduced to absorb unwanted light interference during signal acquisition, improving the signal-to-background noise ratio without affecting the analyte detection intensity, and allowing for the detection of fluorescence emitted by control fluorophores.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If signal acquisition is performed in the presence of a liquid phase to maintain stable and reproducible signal emission, then the signal stability is improved, but light interference increases causing background noise and false results
Solution Approach 1:
The patent introduces absorbent particles as an intermediary substance between the light source and the detection system. These particles absorb unwanted light in the liquid phase while allowing the chemiluminescent signal to pass through, thereby mediating between the need for liquid-phase signal stability and the need to reduce light interference. The absorbent particles specifically target and absorb stray light and reflections without interfering with the chemiluminescent emission from the analyte.
2Measurement precision
If absorbent particles are added to reduce light interference, then background noise is reduced, but the complexity of the analysis method increases
Solution Approach 1:
The patent utilizes the optical properties of absorbent particles that selectively absorb certain wavelengths of light while allowing others to pass through. The particles are chosen or engineered to have specific absorption characteristics that match the unwanted light interference patterns (such as reflections and stray light) without absorbing the chemiluminescent signal wavelengths. This wavelength-selective absorption reduces background noise while maintaining signal integrity, improving measurement precision without requiring complex additional equipment.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach enhances the reliability of analyte detection by reducing background noise, thereby preventing false positives and negatives, and allows for precise quantification of analytes in multiplex analysis methods, even in the presence of a liquid phase.
Implementation Method 1
The use of absorbent particles, such as carbon particles, is introduced to absorb unwanted light interference during signal acquisition, improving the signal-to-background noise ratio
Implementation Method 2
Document U.S. Pat. No. 4,318,707 describes a method for detecting an analyte in a specimen comprising placing the specimen in the presence of an absorbent particle coupled to a first member of a ligand/anti-ligand pair
Data Source
AI summary
The present invention relates to the use of absorbent particles to improve the detection of a signal corresponding to the presence of an analyte in an analysis method on spot(s), in particular when the signal detection takes place in the presence of a liquid phase. The present invention also relates to an analysis method on spot(s) making it possible to improve the detection of a signal corresponding to the presence of an analyte, in the presence of a liquid phase comprising absorbent particles.