ABST Polypeptide Expression for Abiotic Stress Tolerance
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Solution Overview
Problem
Current methods for increasing abiotic stress tolerance in plants are inefficient, time-consuming, and unpredictable, and existing genetic engineering efforts have limitations in generating plants tolerant to a wide range of abiotic stress conditions.
Innovation Solution
Expression of exogenous polynucleotides encoding polypeptides with amino acid sequences at least 90% homologous to specific sequences (e.g., SEQ ID NO: 2, 4, 6, 8, 10, 12, 13-56, 58-63, 66-119, 139-154 or 155) within plants to enhance tolerance to abiotic stresses such as salinity, water deprivation, low temperature, and high temperature, and to increase biomass and yield.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional breeding and traditional genetic engineering methods are used to increase abiotic stress tolerance, then some stress resistance may be achieved, but the process is inefficient, time-consuming, and unpredictable with limited success across multiple stress conditions
Solution Approach 1:
The invention identifies and characterizes specific abiotic stress tolerance genes (ABST genes) and their polypeptide products in advance, creating a ready-to-use genetic resource library. This preliminary identification and characterization of genes with known stress tolerance functions allows for direct application in multiple plant species without repeated screening and selection processes, thereby resolving the contradiction between achieving reliable stress tolerance and the time required for conventional breeding and genetic engineering.
2Reliability
If conventional breeding and genetic engineering are used, then some stress resistance may be obtained, but the methods are inefficient and have unpredictable outcomes
Solution Approach 1:
The invention changes the approach from traditional phenotype-based selection to genotype-based transformation by utilizing identified ABST genes and their polypeptide sequences. By transforming plants with exogenous polynucleotides encoding polypeptides having specific amino acid sequence homology (at least 70%, 80%, 90%, or 95% identical to SEQ ID NOs: 2, 4, 6, 8, 10, 12, 13-56, 58-63, 66-119, 139-154 or 155), the method achieves predictable and reliable stress tolerance outcomes, resolving the contradiction between reliability and productivity in stress tolerance development.
3Adaptability or versatility
If existing genetic engineering efforts are applied, then some tolerance may be achieved, but the scope of stress conditions covered is limited
Solution Approach 1:
The invention identifies ABST genes and polypeptides that provide universal protection against multiple abiotic stress conditions including salinity, drought, flooding, suboptimal temperatures, and toxic chemical pollution. By transforming plants with exogenous polynucleotides encoding polypeptides with high sequence homology to the identified ABST gene products, a single genetic intervention can confer broad-spectrum stress tolerance across diverse environmental challenges, resolving the contradiction between versatility and reliability in stress tolerance.
Data Source
AI summary
Isolated polynucleotides having a nucleic acid sequence at least 80% homologous to SEQ ID NO:1, 3, 5, 7, 9, 11, 158, 159, 160, 161, 162-204, 206-211, 214-287 and/or encoding polypeptides having an amino acid sequence at least 80% homologous to SEQ ID NO: 2, 4, 6, 8, 10, 12, 13-56, 58-63, 66-121, 141-156 or 157 are provided. Also provided are methods of utilizing same for increasing the tolerance of a plant to abiotic stresses and/or increasing the biomass, vigor and/or yield of a plant.