Alternating-Current Voltage Control for Cell Viability
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods lack a controlled and drug-free way to induce anti-apoptotic and anti-necrotic effects in living cells, with the mechanism of using electric currents remaining unclear.
Innovation Solution
Applying an alternating-current voltage within a specific range (25 μA to 75 μA) to living cells, preferably using a device with a conductive stage member and an electric transformer, to induce anti-apoptotic and anti-necrotic effects without administering drugs.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If drugs are administered to induce anti-apoptotic or anti-necrotic effects, then the anti-apoptotic or anti-necrotic effect is achieved, but the complexity of the treatment process increases and potential side effects arise
Solution Approach 1:
The patent replaces the chemical mechanism of drug administration with a physical mechanism of electric current application. By applying an alternating current of specific parameters (10-5000 V, 1-1000 mA), the invention achieves anti-apoptotic and anti-necrotic effects without using drugs, thereby simplifying the treatment process and eliminating drug-related side effects.
2Ease of manufacture
If an electric current is applied to induce anti-apoptotic effect, then the anti-apoptotic effect is achieved without drugs, but the mechanism remains unclear and control precision is insufficient
Solution Approach 1:
The patent establishes specific parameter ranges for electric current application (voltage: 10-5000 V, current: 1-1000 mA, frequency: 50-60 Hz) to achieve optimal anti-apoptotic effects. By defining these precise parameters, the invention enables controlled and reproducible treatment outcomes while maintaining the drug-free approach.
3Reliability
If high voltage is applied to suppress oxidation of food, then the freshness is maintained, but the mechanism remains unclear and safety concerns arise
Solution Approach 1:
The patent applies electric current locally to living cells under controlled conditions (in vitro cell culture or localized tissue application) rather than applying high voltage broadly to food. The specific parameter control (10-5000 V, 1-1000 mA) ensures that the treatment is safe and effective for biological tissues while avoiding the safety risks associated with broad high-voltage application to food.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method effectively induces anti-apoptotic and anti-necrotic effects in living cells with precise control, as demonstrated by enhanced gene expression and reduced apoptosis/necrosis ratios in cultured cells, with optimal results at 50 μA for 24 hours.
Implementation Method 1
applying an alternating-current voltage to a stage member on which a container holding the living cell is placed
Data Source
AI summary
Disclosed herein is a method for simply inducing an anti-apoptotic effect and/or an anti-necrotic effect in a living cell with good control without administering any drug. The method includes applying an alternating-current voltage to the living cell so that an electric current of 25 μA or higher but 75 μA or lower flows to induce an anti-apoptotic effect and/or an anti-necrotic effect in the living cell. The living cell used may be a cultured cell. The alternating-current voltage may be applied to a stage member on which a container holding the living cell is placed.