Accuracy Management Slide for Automatic Immunostaining Fault Diagnosis
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Solution Overview
Problem
Existing automatic immunostaining devices face challenges in determining the type of abnormality when an issue occurs, requiring manual inspections that are labor-intensive.
Innovation Solution
An accuracy management slide with specific spots (antigen (-), fixation (-)/antigen (+), and fixation (+)/antigen (+)) to check for abnormalities and estimate their causes by analyzing color patterns.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If a simple accuracy management slide with one binding substance spot and one non-binding substance spot is used, then the device complexity is reduced, but the measurement precision of abnormality detection deteriorates because the type of abnormality cannot be determined
Solution Approach 1:
The accuracy management slide is segmented into multiple functional spots: a non-binding substance spot (antigen (-)), a binding substance spot without fixation (antigen (+), fixation (-)), and a binding substance spot with fixation (antigen (+), fixation (+)). Each spot serves as an independent diagnostic element that provides specific information about different process steps, enabling precise abnormality classification while maintaining simple overall structure.
Solution Approach 2:
Different spots on the slide are given different local qualities by varying the presence of binding substances and fixation treatments. This creates a gradient of diagnostic capabilities across the slide, where each spot's specific combination of binding substance and fixation status provides unique diagnostic information about particular immunostaining process steps.
2Reliability
If manual inspection by user and periodic inspection by engineer are performed, then the reliability of abnormality detection is improved, but the loss of time and productivity deteriorates due to increased workload
Solution Approach 1:
The accuracy management slide performs self-diagnosis by visually indicating the status of different immunostaining process steps through color development patterns. The slide automatically provides diagnostic information about binding substance function and fixation effectiveness, eliminating the need for complex manual inspection procedures and reducing both time and expertise requirements for abnormality detection.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the detection of abnormalities and their causes in automatic immunostaining devices, ensuring the process proceeds normally and allowing for timely corrective actions.
Implementation Method 1
The staining technique is used for detecting a specific antigen expressed in a specimen with an antibody reagent. Since localization of the antigen to be examined is visualized, immunostaining is an extremely important technique in pathological examination.
Implementation Method 2
When the spot containing a binding substance develops a color and the spot not containing the binding substance does not develop a color, it can be determined that the automatic immunostaining device is normally working.
Data Source
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AI summary
Provided is an accuracy management slide that can check the presence or absence of an abnormality in an automatic immunostaining device and estimate a cause of the abnormality if occurs. The slide includes a spot (antigen (-)) not containing an antigen); a spot (fixation (-)/antigen (+)) having no histological fixation applied and containing an antigen; and a spot (fixation (+)/antigen (+)) having histological fixation applied and containing an antigen.