ACe1 Protein Expression for Corn Borer Control

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Solution Overview

Problem

Current methods for controlling Ostrinia furnacalis (corn borer) damage to plants, including agricultural, chemical, physical, and biological controls, are limited by inefficiency, environmental pollution, and instability, with a need for a more effective and sustainable solution.

Innovation Solution

Production of transgenic plants expressing the ACe1 protein, which inhibits the growth or causes death of Ostrinia furnacalis by contact, providing a comprehensive and stable control method across all growth stages and plant parts.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If chemical pesticides are used to control Ostrinia furnacalis, then the immediate killing effect on larvae is improved, but environmental pollution and pesticide residue increase

Engineering Contradiction:
Improvekilling effectVSAvoidenvironmental pollution
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent converts the harmful effect of insecticides into a beneficial one by using genetically engineered plants that produce insecticidal proteins (Cry1Ab, Cry2Ab, Vip3A) internally. These proteins specifically target and kill corn borer larvae while being environmentally friendly, eliminating the need for external chemical pesticide application and thus resolving the contradiction between effective pest control and environmental protection

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

Solution Approach 2:

The transgenic plants are designed to self-produce insecticidal proteins through genetic modification, enabling them to protect themselves against Ostrinia furnacalis infestation without requiring external chemical interventions. This self-service mechanism provides continuous protection throughout the plant's growth cycle while avoiding environmental pollution associated with repeated pesticide applications

Inventive Principle:
Principle #25Self-service

2Object-affected harmful factors

If agricultural control methods are used, then environmental friendliness is improved, but control effectiveness and stability deteriorate

Engineering Contradiction:
Improveenvironmental friendlinessVSAvoidcontrol effectiveness
Core Design Contradiction:
Object-affected harmful factorsVSReliability

Solution Approach 1:

The transgenic plants autonomously produce insecticidal proteins throughout their growth cycle, providing consistent and reliable protection against corn borer without requiring external agricultural interventions. This self-service capability ensures stable control effectiveness while maintaining environmental friendliness, as the plants actively defend themselves against pests rather than relying on periodic human-applied treatments

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The genetic modification enables continuous production of insecticidal proteins throughout the plant's entire growth period, from seedling to maturity. This continuous protective action eliminates the gaps and inconsistencies inherent in periodic agricultural control methods, ensuring reliable pest suppression while maintaining environmental sustainability

Inventive Principle:
Principle #20Continuity of useful action

3Stability of the object's composition

If transgenic plants expressing ACe1 protein are produced, then control stability across all growth stages is improved, but the complexity of plant production increases

Engineering Contradiction:
Improvecontrol stabilityVSAvoidplant production complexity
Core Design Contradiction:
Stability of the object's compositionVSDevice complexity

Solution Approach 1:

The transgenic plants are engineered to express multiple insecticidal proteins (Cry1Ab, Cry2Ab, Vip3A, and ACe1) simultaneously, creating a universal solution that provides broad-spectrum protection against Ostrinia furnacalis across all growth stages and plant parts. This multi-functional approach consolidates what would otherwise require multiple separate control measures into a single integrated system, managing complexity while enhancing stability

Inventive Principle:
Principle #6Universality (Multi-functionality)

Solution Approach 2:

The patent combines multiple insecticidal protein genes into a single transgenic plant system, creating a composite biological material that integrates the protective functions of different proteins. This composite approach achieves stable, multi-stage protection while streamlining the production process compared to applying multiple separate treatments

Inventive Principle:
Principle #40Composite materials

Data Source

PatentUS20230180767A1Use of insecticidal protein
Publication Date: 2023.06.15 BEIJING DABEINONG BIOTECHNOLOGY CO LTD
  • US20230180767A1 patent drawing

AI summary

Related is a use of an insecticidal protein. The insecticidal protein may be used to control Ostrinia furnacalis (Hubern). A method for controlling Ostrinia furnacalis (Hubern) includes: allowing the Ostrinia furnacalis (Hubern) to be at least in contact with an ACe1 protein. In the present application, the ACe1 protein that can kill the Ostrinia furnacalis (Hubern) is produced in bacteria and/or a plant body to control the Ostrinia furnacalis (Hubern).