Acellular ECM Composite for Tissue Regeneration

Resolve Bottlenecks,
Find Innovative SolutionsGenerate Solutions

Solution Overview

Problem

Current medical graft materials derived from extracellular matrix (ECM) lack improved alternatives for enhanced tissue growth and cell culture applications, with existing materials often retaining cells or cell components that can be immunogenic and limiting their effectiveness.

Innovation Solution

A medical graft material comprising a substantially acellular extracellular matrix composite is developed, where a first ECM material is combined with a second biosynthetically-deposited ECM material, non-native to the first, to create a composite devoid of cells and cell components, promoting endogenous tissue growth and cell culture by decellularizing the ECM composite after cell deposition.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If existing ECM graft materials are used, then tissue growth and cell culture applications are supported, but cell components remain that can be immunogenic and limit effectiveness

Engineering Contradiction:

Improvetissue growth supportVSAvoidimmunogenic cell components

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The patent applies decellularization processes to extract and remove all cellular components from the ECM graft material, leaving only the extracellular matrix framework. This extraction eliminates immunogenic cell components while preserving the structural and functional properties needed for tissue growth and cell culture applications.

Inventive Principle:

Principle #2Taking out (Extraction)

Solution Approach 2:

The patent uses decellularization agents and processing steps as intermediaries to facilitate the removal of cell components. These intermediaries enable the transformation of cellular ECM into acellular composite materials while maintaining the essential matrix structure for biological activity.

Inventive Principle:

Principle #24Intermediary (Mediator)

2Ease of manufacture

If a single ECM material is used, then manufacturing is simple, but enhanced tissue growth and reduced inflammatory responses are limited

Engineering Contradiction:

ImproveECM material processingVSAvoidtissue integration

Core Design Contradiction:

Ease of manufactureVSReliability

Solution Approach 1:

The patent creates composite ECM materials by combining a first ECM material with a second biosynthetically-deposited ECM material. This composite structure enhances tissue growth and reduces inflammatory responses while maintaining manufacturability through a systematic two-step process of deposition and decellularization.

Inventive Principle:

Principle #40Composite materials

Solution Approach 2:

The patent performs biosynthetic deposition of the second ECM material onto the first ECM material before decellularization. This preliminary action ensures that the enhanced functional properties are embedded in the matrix structure in advance, allowing the final acellular composite to exhibit improved tissue integration without complicating the overall manufacturing process.

Inventive Principle:

Principle #10Preliminary action

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The composite material enhances cell attachment, metabolic activity, and reduces inflammatory responses, improving tissue integration and reducing platelet adhesion, thereby supporting effective tissue regeneration and cell growth without immunogenic reactions.

Implementation Method 1

culturing cells in vitro under conditions to secrete extracellular matrix components wherein said components are transferred to said surface to form an extracellular matrix composite

Methodology Applied

Scientific EffectBiosynthetic deposition:

Implementation Method 2

decellularizing the extracellular matrix composite

Methodology Applied

Scientific EffectDecellularization:

Data Source

PatentUS9186435B2Extracellular matrix composite materials, and manufacture and use thereof

Publication Date: 2015.11.17 COOK BIOTECH INC

AI summary

Described are preferred extracellular matrix composites including a first extracellular matrix material having a second extracellular matrix material deposited thereon. The preferred materials are made by culturing cells in contact with an extracellular matrix graft material in a fashion to cause the cells to biosynthesize and deposit extracellular matrix components on the material. The cells are then removed to provide the extracellular matrix composite material.