3D-Printed Acellular Matrix Woven Scaffolds for Cell Penetration
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Solution Overview
Problem
Existing acellular matrix scaffold materials face challenges in achieving fine control of material structure for effective cell loading and tissue repair, with limitations in cell penetration, mechanical properties, and scalability, particularly in producing long fibers suitable for large tissue defects.
Innovation Solution
A method combining 3D printing with weaving to prepare acellular matrix woven materials, involving decellularization, rotary cutting, twisting, and precise structural design to create long fibers with controlled macro and microstructures, using peracetic acid and sodium dodecyl sulfate for decellularization, and 3D printing to weave plain, twill, or satin weave patterns.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If acellular matrix material is obtained by direct decellularization of tissues, then good biocompatibility is achieved, but the compact structure makes cell penetration difficult resulting in poor cell migration
Solution Approach 1:
The patent applies porous materials by constructing an acellular matrix scaffold with controlled pore structures through decellularization processes. The scaffold maintains the natural extracellular matrix framework while creating interconnected pores that enable cell penetration and migration, thus preserving biocompatibility while improving cell accessibility.
Solution Approach 2:
The patent applies segmentation by dividing the compact tissue structure into smaller functional units during decellularization. The tissue is processed to create a segmented architecture with distributed pores and channels, allowing cells to penetrate and migrate throughout the material while maintaining overall structural integrity and biocompatibility.
2Manufacturing precision
If conventional weaving methods are used to create 3D scaffold materials, then certain dimensions can be manufactured, but the processes are complicated and difficult to accurately control micro and macro structural shapes
Solution Approach 1:
The patent replaces conventional mechanical weaving processes with a decellularization-based fabrication approach. By using biochemical decellularization methods followed by controlled restructuring, the patent achieves precise control over both micro and macro structural shapes without the complexity of traditional mechanical weaving systems.
Solution Approach 2:
The patent applies parameter changes by controlling various parameters during the decellularization and scaffold construction process, including crosslinking degree, pore size distribution, fiber alignment, and structural density. This enables accurate control of both micro and macro structural characteristics while simplifying the overall manufacturing process.
3Ease of operation
If acellular matrix hydrogels are used for cell loading, then cell loading is enabled, but mechanical properties are weak limiting the effectiveness of cell therapy
Solution Approach 1:
The patent applies composite materials by combining acellular matrix components with reinforcing elements during scaffold construction. The resulting composite structure maintains the cell-loading capability of hydrogels while incorporating structural reinforcements that significantly improve mechanical strength and load-bearing capacity.
Solution Approach 2:
The patent applies segmentation by creating a hierarchical structure where soft hydrogel regions for cell loading are combined with stronger structural frameworks. This segmented architecture allows different regions to fulfill different functions - cell loading and mechanical support - simultaneously.
4Reliability
If natural materials such as collagen, gelatin, silk fibroin and chitosan are used, then good biocompatibility is achieved, but mechanical properties and processability are poor
Solution Approach 1:
The patent applies composite materials by combining natural biocompatible materials with synthetic or semi-synthetic components. This composite approach enhances the mechanical properties and processability of natural materials while preserving their biocompatibility, enabling easier manufacturing and fabrication of scaffolds.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method enhances cell loading efficiency, accelerates cell migration and tissue regeneration, and supports various tissue repairs by providing a biocompatible, porous structure that promotes cell adhesion, proliferation, and differentiation, suitable for diverse tissue engineering applications.
Implementation Method 1
peracetic acid and sodium dodecyl sulfate for decellularization
Implementation Method 2
peracetic acid and sodium dodecyl sulfate for decellularization
Implementation Method 3
rotary cutting
Implementation Method 4
twisting
Implementation Method 5
3D printing to weave plain, twill, or satin weave patterns
Implementation Method 6
weaving
Data Source
AI summary
An acellular matrix woven material, a preparation method and an application thereof are provided. The acellular matrix woven material is prepared by combining 3D printing with weaving. At the same time, also provided are the acellular matrix woven material prepared by the preparation method and its application in preparing tissue engineering scaffold materials.