ACh1 Protein Expression for Monolepta hieroglyphica Control

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Solution Overview

Problem

Current methods for controlling Monolepta hieroglyphica (Motschulsky) infestation in corn and soybean crops, including agricultural, chemical, physical, and biological controls, are ineffective due to the pest's underground habits and resistance to existing insecticides, leading to significant food losses and ecosystem disruptions.

Innovation Solution

Production of transgenic plants expressing the ACh1 protein, which inhibits the growth or causes death of Monolepta hieroglyphica (Motschulsky) when ingested, providing a persistent and environmentally friendly control method across the plant's growth stages.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If chemical control methods are used to control Monolepta hieroglyphica, then pest control effectiveness is improved, but environmental pollution and pesticide residue are worsened

Engineering Contradiction:

Improvepest control effectivenessVSAvoidenvironmental pollution

Core Design Contradiction:

ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The plant produces and expresses the ACh1 protein itself to control the pest, eliminating the need for external chemical pesticides. The plant's own biological system serves the protective function, reducing environmental pollution while maintaining pest control effectiveness.

Inventive Principle:

Principle #25Self-service

Solution Approach 2:

The patent replaces chemical pesticide systems with a biological protein-based system. The ACh1 protein, produced by transgenic plants, provides pest control through biological mechanisms rather than chemical toxins, thereby reducing environmental harm.

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

2Reliability

If seed coating agents are used to control underground larvae, then early stage pest control is improved, but control effectiveness deteriorates due to time and rain environment influence

Engineering Contradiction:

Improveearly stage pest controlVSAvoidpersistent period

Core Design Contradiction:

ReliabilityVSDuration of action of stationary object

Solution Approach 1:

The plant is genetically engineered to continuously produce the ACh1 protein from the outset, providing preemptive protection against pest infestation throughout the entire growth period. This eliminates the temporary nature of seed coating agents and ensures sustained control effectiveness.

Inventive Principle:

Principle #10Preliminary action

Solution Approach 2:

The transgenic plant continuously expresses the ACh1 protein throughout its growth cycle, providing uninterrupted pest control. This continuous biological protection replaces the temporary protective layer of seed coating agents, maintaining effectiveness regardless of environmental conditions.

Inventive Principle:

Principle #20Continuity of useful action

3Reliability

If broad irrigation is used to drown eggs and larvae in soil, then pest control is improved, but water resource consumption is worsened

Engineering Contradiction:

Improvepest controlVSAvoidwater resource consumption

Core Design Contradiction:

ReliabilityVSLoss of substance

Solution Approach 1:

The plant uses its own expressed ACh1 protein to control pests, eliminating the need for water-based irrigation methods. This biological control mechanism provides pest management without consuming additional water resources.

Inventive Principle:

Principle #25Self-service

4Speed

If chemical spraying is used to control adult pests, then rapid pest reduction is improved, but implementation complexity is worsened due to migration between fields

Engineering Contradiction:

Improverapid pest reductionVSAvoidorganizational management complexity

Core Design Contradiction:

SpeedVSDevice complexity

Solution Approach 1:

Each plant individually produces and expresses the ACh1 protein, providing localized pest control without requiring coordinated regional spraying operations. This eliminates the complex organizational management needed for unified chemical control while maintaining rapid pest reduction through biological means.

Inventive Principle:

Principle #25Self-service

Data Source

PatentUS20230212602A1Use of insecticidal protein

Publication Date: 2023.07.06 BEIJING DABEINONG BIOTECHNOLOGY CO LTD

AI summary

Related is a use of an insecticidal protein. The insecticidal protein may be used to control Monolepta hieroglyphica (Motschulsky). A method for controlling the Monolepta hieroglyphica (Motschulsky) includes: allowing the Monolepta hieroglyphica (Motschulsky) to be at least in contact with an ACh1 protein. In the present application, the Monolepta hieroglyphica (Motschulsky) is controlled through producing the ACh1 protein that can kill the Monolepta hieroglyphica (Motschulsky) in bacteria and/or a plant in vivo.