Acid-Base Treated Polyamide Media Balancing Protein Recovery and Strength
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Solution Overview
Problem
Existing methods for removing contaminants from protein-containing solutions, such as those using ion exchange columns and size exclusion mechanisms, result in the adsorption of target proteins, reducing their recovery rate, and the polyamide media lose strength when treated with acids or alkalis.
Innovation Solution
A method involving treating a polyamide medium with acidic or alkaline aqueous solutions to alter its surface properties, specifically using solutions with pH 5 or less for acids and pH 10 or more for alkalis, to produce a polyamide medium that maintains strength and reduces target protein adsorption, thereby enhancing recovery rates.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If conventional adsorption methods (ion exchange column or hydrophobic gel) are used to remove contaminants, then contaminant removal is achieved, but target protein adsorption also occurs reducing recovery rate
Solution Approach 1:
The patent applies parameter changes by treating the polyamide medium with acidic or alkaline aqueous solutions to modify its surface properties. This treatment alters the charge distribution and hydrophilicity of the polyamide medium, enabling it to selectively adsorb contaminants while minimizing target protein adsorption, thus resolving the contradiction between contaminant removal and protein recovery rate
Solution Approach 2:
The patent applies local quality by creating non-uniform charge distribution within the polyamide medium through acid-base treatment. The treatment generates both positive and negative charge regions locally within the medium structure, allowing different regions to selectively interact with different types of contaminants while maintaining low non-specific protein binding
2Loss of substance
If polyamide medium is treated with strong acid or base to modify surface properties, then protein adsorption is reduced, but medium strength deteriorates
Solution Approach 1:
The patent applies parameter changes by carefully controlling the pH range and treatment conditions of the acid-base treatment. By using mild acidic or alkaline aqueous solutions with controlled concentration and exposure time, the surface properties of the polyamide medium are modified to reduce protein adsorption while avoiding excessive degradation that would compromise medium strength
Solution Approach 2:
The patent applies continuity of useful action by implementing a sequential treatment process where the polyamide medium undergoes controlled acid-base treatment followed by proper washing and stabilization. This continuous process ensures that the beneficial surface modification is achieved while maintaining structural integrity through proper process control
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The treated polyamide medium achieves a higher recovery rate of target proteins and maintains its strength, effectively purifying protein-containing solutions, particularly those with antibody aggregates, by selectively removing contaminants while minimizing protein adsorption.
Implementation Method 1
a method using an ion exchange column or a hydrophobic gel utilizing an adsorption mechanism
Implementation Method 2
a method for removing a contaminant using a size exclusion mechanism has proposed, including a method using a size exclusion column
Data Source
AI summary
A method for producing a polyamide medium for purifying a protein-containing solution, comprising: a step of treating a polyamide medium before a treatment with an acidic or alkaline aqueous solution, with an acidic or alkaline aqueous solution.